School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, UK.
ISME J. 2012 Jan;6(1):223-6. doi: 10.1038/ismej.2011.79. Epub 2011 Jun 16.
Ruegeria pomeroyi DSS-3 is a model Roseobacter marine bacterium, particularly regarding its catabolism of dimethylsulfoniopropionate (DMSP), an abundant anti-stress molecule made by marine phytoplankton. We found a novel gene, dddW, which encodes a DMSP lyase that cleaves DMSP into acrylate plus the environmentally important volatile dimethyl sulfide (DMS). Mutations in dddW reduced, but did not abolish DMS production. Transcription of dddW was greatly enhanced by pre-growth of cells with DMSP, via a LysR-type regulator. Close DddW homologs occur in only one other Roseobacter species, and there are no close homologs and only a few related sequences in metagenomes of marine bacteria. In addition to DddW, R. pomeroyi DSS-3 had been shown to have two other, different, DMSP lyases, DddP and DddQ, plus an enzyme that demethylates DMSP, emphasizing the importance of this substrate for this model bacterium.
鲁氏红杆菌 DSS-3 是一种模式玫瑰杆菌海洋细菌，特别是在其分解二甲硫醚（DMSP）方面，DMSP 是一种由海洋浮游植物产生的丰富抗应激分子。我们发现了一个新基因 dddW，它编码一种 DMSP 裂解酶，可将 DMSP 裂解为丙烯酸盐和环境重要的挥发性二甲硫（DMS）。dddW 的突变减少了 DMS 的产生，但没有完全消除。通过 LysR 型调节剂，细胞在 DMSP 预生长时大大增强了 dddW 的转录。只有另一种玫瑰杆菌物种中存在与 DddW 密切相关的同源物，而在海洋细菌的宏基因组中没有密切相关的同源物，只有少数相关序列。除了 DddW，R. pomeroyi DSS-3 还被证明具有另外两种不同的 DMSP 裂解酶 DddP 和 DddQ，以及一种将 DMSP 去甲基化的酶，这强调了这种底物对这种模式细菌的重要性。
