Wang M, Tang S T, Lund O, Dziegiel M H, Buus S, Claesson M H
Department of International Health, Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, Copenhagen, The Netherlands.
Clin Exp Immunol. 2009 Mar;155(3):441-6. doi: 10.1111/j.1365-2249.2008.03856.x.
Interferon-gamma secreting T lymphocytes against pox virus-derived synthetic 9-mer peptides were tested by enzyme-linked immunospot in peripheral blood of individuals vaccinated with vaccinia virus more than 30 years ago. The peptides were characterized biochemically as high-affinity human leucocyte antigen (HLA) class I binders (K(D) <or= 5 nM). However, five of the individuals tested did not show typical CD8(+) T cell-mediated HLA class I-restricted responses. Instead, these donors showed CD4(+) T cell-dependent responses against four of a total of eight antigenic 9-mer peptides discovered recently by our group. These latter responses were blocked specifically in the presence of anti-HLA class II antibody. We conclude that long-lived memory responses against pox virus-derived 9-mer peptides, with high binding affinity for HLA class I molecules, are mediated in some cases by CD4(+) T cells and apparently restricted by HLA class II molecules.
在30多年前接种过牛痘病毒的个体外周血中，通过酶联免疫斑点法检测了针对痘病毒衍生的合成9肽的干扰素-γ分泌性T淋巴细胞。这些肽在生化上被鉴定为高亲和力人类白细胞抗原（HLA）I类结合物（K（D）≤5 nM）。然而，所检测的个体中有5人未表现出典型的CD8（+）T细胞介导的HLA I类限制性反应。相反，这些供体对我们小组最近发现的总共8种抗原性9肽中的4种表现出CD4（+）T细胞依赖性反应。在抗HLA II类抗体存在的情况下，这些反应被特异性阻断。我们得出结论，针对对HLA I类分子具有高结合亲和力的痘病毒衍生9肽的长期记忆反应，在某些情况下由CD4（+）T细胞介导，并且显然受HLA II类分子限制。
