Jaeschke Andrea, Op den Camp Huub J M, Harhangi Harry, Klimiuk Adam, Hopmans Ellen C, Jetten Mike S M, Schouten Stefan, Sinninghe Damsté Jaap S
Department of Marine Organic Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, Texel, The Netherlands.
FEMS Microbiol Ecol. 2009 Mar;67(3):343-50. doi: 10.1111/j.1574-6941.2008.00640.x.
Anammox, the oxidation of ammonium with nitrite to dinitrogen gas under anoxic conditions, is an important process in mesophilic environments such as wastewaters, oceans and freshwater systems, but little is known of this process at elevated temperatures. In this study, we investigated anammox in microbial mats and sediments obtained from several hot springs in California and Nevada, using geochemical and molecular microbiological methods. Anammox bacteria-specific ladderane core lipids with concentrations ranging between 0.3 and 52 ng g(-1) sediment were detected in five hot springs analyzed with temperatures up to 65 degrees C. In addition, 16S rRNA gene analysis showed the presence of genes phylogenetically related to the known anammox bacteria Candidatus Brocadia fulgida, Candidatus Brocadia anammoxidans and Candidatus Kuenenia stuttgartiensis (96.5-99.8% sequence identity) in three hot springs with temperatures up to 52 degrees C. Our data indicate that anammox bacteria may be able to thrive at thermophilic temperatures and thus may play a significant role in the nitrogen cycle of hot spring environments.
厌氧氨氧化是指在缺氧条件下将铵与亚硝酸盐氧化为氮气的过程，在诸如废水、海洋和淡水系统等中温环境中是一个重要过程，但在高温条件下对该过程的了解却很少。在本研究中，我们使用地球化学和分子微生物学方法，对从加利福尼亚州和内华达州的几个温泉中获取的微生物垫和沉积物中的厌氧氨氧化进行了研究。在分析的五个温度高达65℃的温泉中，检测到了厌氧氨氧化细菌特有的梯烷核心脂质，其浓度在0.3至52 ng g(-1)沉积物之间。此外，16S rRNA基因分析表明，在三个温度高达52℃的温泉中存在与已知厌氧氨氧化细菌“Candidatus Brocadia fulgida”、“Candidatus Brocadia anammoxidans”和“Candidatus Kuenenia stuttgartiensis”系统发育相关的基因（序列同一性为96.5 - 99.8%）。我们的数据表明，厌氧氨氧化细菌可能能够在嗜热温度下茁壮成长，因此可能在温泉环境的氮循环中发挥重要作用。
