从毛栓菌中纯化和鉴定具有独特氧化特性的耐热漆酶
Purification and characterization of a thermostable laccase with unique oxidative characteristics from Trametes hirsuta.
作者信息
Haibo Zhang, Yinglong Zhang, Feng Huang, Peiji Gao, Jiachuan Chen
机构信息
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
出版信息
Biotechnol Lett. 2009 Jun;31(6):837-43. doi: 10.1007/s10529-009-9945-0. Epub 2009 Feb 17.
A laccase was purified from Trametes hirsuta. This laccase was classified as a "white" or "yellow" laccase. pH 2.4 was optimal for the oxidation of ABTS and pH 2.5 for DMP. DMP oxidation was optimal at 85 degrees C. The half-life of this laccase was 70 min at 75 degrees C, and 5 h at 65 degrees C. Non-phenolic dyes, such as Methyl Red, were oxidized by purified laccase without mediators. The enzyme was not inhibited by Cu(2+), Mn(2+), or EDTA. These are atypical laccase characteristics that make it a good candidate for theoretical and applied research.
从毛栓菌中纯化出一种漆酶。这种漆酶被归类为“白色”或“黄色”漆酶。ABTS氧化的最适pH为2.4，DMP氧化的最适pH为2.5。DMP氧化在85℃时最适宜。这种漆酶在75℃时的半衰期为70分钟，在65℃时为5小时。非酚类染料，如甲基红，可被纯化的漆酶在无介体的情况下氧化。该酶不受Cu(2+)、Mn(2+)或EDTA的抑制。这些是非典型的漆酶特性，使其成为理论和应用研究的良好候选对象。
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