毛叶轮环藤叶乙醇提取物对正常大鼠的急性和慢性利尿作用

Acute and chronic diuretic effect of ethanolic extract of leaves of Cocculus hirsutus (L.) Diles in normal rats.

作者信息

Badole Sachin L, Bodhankar Subhash L, Patel Naimesh M, Bhardwaj Subodh

机构信息

Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune, India.

出版信息

J Pharm Pharmacol. 2009 Mar;61(3):387-93. doi: 10.1211/jpp/61.03.0015.

DOI:10.1211/jpp/61.03.0015

PMID:19222913

Abstract

OBJECTIVES

The objective of this study was to evaluate the acute and chronic diuretic effect of the ethanolic extract of the leaves of Cocculus hirsutus (L.) Diles.

METHODS

The ethanolic extract was administered (100, 200 and 400 mg/kg, p.o.) in Wistar rats. In the acute study, rats received drugs orally and urine was collected after 1, 2, 3, 4, 5 and 6 h. The chronic study involved repeated administration of ethanolic extract for 28 days and urine was collected on day 1, 7, 14, 21 and 28. The parameters were total urine volume, concentration of Na+, K+ and Cl- ions, creatinine in urine and serum. Urine output, electrolytes (Na+, K+ and Cl- ions) and creatinine were determined on day 7, 14, 21 and 28.

KEY FINDINGS

The highest dose (400 mg/kg) of the ethanolic extract significantly (P < 0.01) enhanced urine output. Excretion of cations (Na+ and K+ ions) and anions (Cl- ions) increased significantly with respect to the control (gum acacia 2% dissolved in saline, 10 ml/kg) group. The increase of cations in the urine after treatment with ethanolic extract was dose dependent. The ethanolic extract of the leaves of C. hirsutus (100, 200 and 400 mg/kg) and furosemide (10 mg/kg) did not significantly change the concentration of Na+, K+ and Cl- ions in serum. The ethanolic extract of the leaves of C. hirsutus (100, 200 and 400 mg/kg) and furosemide (10 mg/kg) increased the excretion of creatinine in urine but with a corresponding decrease in serum.

CONCLUSIONS

It was concluded that the ethanolic extract of the leaves of C. hirsutus (400 mg/kg) had significant diuretic effect in rats.

摘要

目的

本研究旨在评估毛叶轮环藤（Cocculus hirsutus (L.) Diles）叶乙醇提取物的急性和慢性利尿作用。

方法

将乙醇提取物（100、200和400毫克/千克，口服）给予Wistar大鼠。在急性研究中，大鼠口服药物，并在1、2、3、4、5和6小时后收集尿液。慢性研究涉及乙醇提取物重复给药28天，并在第1、7、14、21和28天收集尿液。参数包括总尿量、Na⁺、K⁺和Cl⁻离子浓度、尿肌酐和血清肌酐。在第7、14、21和28天测定尿量、电解质（Na⁺、K⁺和Cl⁻离子）和肌酐。

主要发现

乙醇提取物的最高剂量（400毫克/千克）显著（P < 0.01）增加尿量。与对照组（2%阿拉伯胶溶解于生理盐水，10毫升/千克）相比，阳离子（Na⁺和K⁺离子）和阴离子（Cl⁻离子）的排泄显著增加。乙醇提取物处理后尿中阳离子的增加呈剂量依赖性。毛叶轮环藤叶乙醇提取物（100、200和400毫克/千克）和呋塞米（10毫克/千克）对血清中Na⁺、K⁺和Cl⁻离子浓度无显著影响。毛叶轮环藤叶乙醇提取物（100、200和400毫克/千克）和呋塞米（10毫克/千克）增加尿肌酐排泄，但血清肌酐相应降低。