Van Cann E M, Slootweg P J, de Wilde P C M, Otte-Höller I, Koole R, Stoelinga P J W, Merkx M A W
Department of Oral and Maxillofacial Surgery, University Medical Centre Utrecht, Utrecht, The Netherlands.
Int J Oral Maxillofac Surg. 2009 Mar;38(3):279-84. doi: 10.1016/j.ijom.2009.01.005. Epub 2009 Feb 14.
Destruction of bone by tumour is caused by osteoclasts rather than by tumour cells directly. Tumour cells of invasive oral squamous cell carcinomas (SCC) release osteoclast-related cytokines and cytokines activate osteoclasts. The purpose of this study was to investigate the possibility of predicting mandibular invasion by SCC by analysis of the expression of osteoclast-related cytokines in biopsy specimens of SCC, adjacent or fixed to the mandible. Thirty-five biopsy specimens from the pathology archives were examined from patients who had been treated for SCC, adjacent or fixed to the mandible. The patients were divided into those with and without medullary invasion. The expression of tumour necrosis factor (TNF)-alpha, interleukin (IL)-6 and IL-11 was studied by immunohistochemical analysis. No significant differences were found in expression of TNF-alpha, IL-6 and IL-11 between biopsy specimens with or without medullary invasion. Quantification of the density of tumour-infiltrating lymphocytes was not reproducible. In conclusion, the expression of TNF-alpha, IL-6 and IL-11 in biopsy specimens of SCC, adjacent or fixed to the mandible, is not an appropriate method for predicting the presence of medullary invasion of the mandible.
肿瘤对骨的破坏是由破骨细胞引起的，而非肿瘤细胞直接导致。浸润性口腔鳞状细胞癌（SCC）的肿瘤细胞释放与破骨细胞相关的细胞因子，这些细胞因子激活破骨细胞。本研究的目的是通过分析SCC活检标本（紧邻或附着于下颌骨）中破骨细胞相关细胞因子的表达，探讨预测SCC下颌骨浸润的可能性。从病理档案中选取了35例接受过紧邻或附着于下颌骨的SCC治疗患者的活检标本进行检查。患者被分为有髓质浸润和无髓质浸润两组。通过免疫组织化学分析研究肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6和IL-11的表达。在有或无髓质浸润的活检标本中，TNF-α、IL-6和IL-11的表达未发现显著差异。肿瘤浸润淋巴细胞密度的定量分析结果不可重复。总之，紧邻或附着于下颌骨的SCC活检标本中TNF-α、IL-6和IL-11的表达，并非预测下颌骨髓质浸润存在与否的合适方法。
