Huebner Ansgar, Bratton Dan, Whyte Graeme, Yang Min, Demello Andrew J, Abell Chris, Hollfelder Florian
Department of Biochemistry, University of Cambridge, 80, Tennis Court Road, Cambridge, UK CB2 1GA.
Lab Chip. 2009 Mar 7;9(5):692-8. doi: 10.1039/b813709a. Epub 2008 Nov 27.
We describe the design, fabrication and use of a single-layered poly(dimethylsiloxane) microfluidic structure for the entrapment and release of microdroplets in an array format controlled entirely by liquid flow. Aqueous picoliter droplets are trapped en masse and optically monitored for extended periods of time. Such an array-based approach is used to characterize droplet shrinkage, aggregation of encapsulated E. coli cells and enzymatic reactions. We also demonstrate that trapped droplets may be recovered from the microfluidic array for further processing.
我们描述了一种单层聚二甲基硅氧烷微流体结构的设计、制造和使用，该结构用于以完全由液体流动控制的阵列形式捕获和释放微滴。皮升大小的水滴被大量捕获，并进行长时间的光学监测。这种基于阵列的方法用于表征水滴收缩、封装的大肠杆菌细胞的聚集以及酶促反应。我们还证明，可以从微流体阵列中回收捕获的水滴以进行进一步处理。
