Wang Yaohui, Zheng Haiyan, Liu Shengyuan
Department of Pathophysiology, Zunyi Medical College, Zunyi, China.
J Huazhong Univ Sci Technolog Med Sci. 2009 Feb;29(1):8-11. doi: 10.1007/s11596-009-0102-8. Epub 2009 Feb 18.
This study investigated the effects of ATP-sensitive potassium channels on the expression of P21, P27 and leptin. The expression of receptor of ATP-sensitive potassium channels (sulphonylurea receptor, SUR) mRNA in the preadipocytes and leptin mRNA was detected by PCR after rat preadipocytes were treated with the opener (diazoxide) or inhibitor (glibenclamide) of ATP-sensitive potassium channels during the process of inducing differentiation. The expression of P21 and P27 in preadipocytes treated with diazoxide or glibenclamide was assayed by Western blot. The results showed that the expression of SUR2, not SUR1 was detected in adipose tissue, preadipocytes and adipocytes. After treatment of preadipocytes with diazoxide, the expression levels of P21 and P27 were obviously higher than those in control group, but the expression levels of P21 and P27 in glibenclamide-treated group were lower than those in control group. During the process of inducing differentiation, the expression of leptin mRNA in preadipocytes treated with diazoxide was increased greatly, but the expression of leptin mRNA in glibenclamide-treated group decreased obviously. It was concluded that ATP-sensitive potassium channels might be involved in the proliferation and differentiation of rat preadipocytes by changing the expression of P21, P27 and leptin.
本研究探讨了ATP敏感性钾通道对P21、P27和瘦素表达的影响。在大鼠前脂肪细胞诱导分化过程中，用ATP敏感性钾通道开放剂（二氮嗪）或抑制剂（格列本脲）处理后，采用PCR检测前脂肪细胞中ATP敏感性钾通道受体（磺酰脲受体，SUR）mRNA及瘦素mRNA的表达。采用蛋白质免疫印迹法检测经二氮嗪或格列本脲处理的前脂肪细胞中P21和P27的表达。结果显示，在脂肪组织、前脂肪细胞和脂肪细胞中检测到的是SUR2而非SUR1的表达。用二氮嗪处理前脂肪细胞后，P21和P27的表达水平明显高于对照组，而格列本脲处理组中P21和P27的表达水平低于对照组。在诱导分化过程中，二氮嗪处理的前脂肪细胞中瘦素mRNA的表达显著增加，而格列本脲处理组中瘦素mRNA的表达明显降低。结论：ATP敏感性钾通道可能通过改变P21、P27和瘦素的表达参与大鼠前脂肪细胞的增殖和分化。
