Jiang Ke, Cheng Lin, Wang Jiangjun, Li Jinsong, Nie Jun
Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
J Huazhong Univ Sci Technolog Med Sci. 2009 Feb;29(1):84-7. doi: 10.1007/s11596-009-0118-0. Epub 2009 Feb 18.
This study investigated the expression of hemeoxygenase-1 (HO-1) in rats with acute lung rejection and its implication. A valid rat orthotopic left lung transplantation model (SD rat-->Wistar rat) was established by using an improved three-cuff anastomosis technique. The rats were divided into control group, CoPP (HO-1 inducer)-treated group and ZnPP (HO-1 inhibitor)- treated group. The severity of acute rejection was graded on the basis of the morphologic changes of the lung samples stained with HE. The expression of HO-1 protein in lung tissue was detected by using immunohistochemistry and Western blot, and HO-1 mRNA activity was assayed by RT-PCR. The results showed that the expression of HO-1 protein was significantly increased with the acute rejection grading in rats (P<0.01). As compared with control and ZnPP-treated groups, the severity of acute rejection was not alleviated and the grade not reduced significantly in CoPP-treated group (P>0.05). It was concluded that HO-1 protein might be involved in the pathological process of post-graft acute rejection. The expression of HO-1 protein was increased gradually with aggravation of acute rejection, and HO-1 protein might be used as an index to monitor acute rejection after lung transplantation.
本研究探讨血红素加氧酶-1（HO-1）在大鼠急性肺排斥反应中的表达及其意义。采用改良的三袖套吻合技术建立有效的大鼠原位左肺移植模型（SD大鼠→Wistar大鼠）。将大鼠分为对照组、CoPP（HO-1诱导剂）处理组和ZnPP（HO-1抑制剂）处理组。根据HE染色的肺组织形态学变化对急性排斥反应的严重程度进行分级。采用免疫组织化学和蛋白质印迹法检测肺组织中HO-1蛋白的表达，采用RT-PCR法检测HO-1 mRNA活性。结果显示，大鼠急性排斥反应分级时HO-1蛋白表达显著增加（P<0.01）。与对照组和ZnPP处理组相比，CoPP处理组急性排斥反应的严重程度未减轻，分级也未显著降低（P>0.05）。结论：HO-1蛋白可能参与移植后急性排斥反应的病理过程。HO-1蛋白表达随急性排斥反应加重而逐渐增加，HO-1蛋白可作为监测肺移植后急性排斥反应的指标。
