用偏振光刺激环糊精葡萄糖基转移酶形成环糊精。
Formation of cyclodextrins with cyclodextrin glucosyltransferase stimulated with polarized light.
作者信息
Fiedorowicz Maciej, Khachatryan Gohar, Konieczna-Molenda Anna, Tomasik Piotr
机构信息
Dept. of Chemistry, University of Agriculture, Cracow 30-149, Poland.
出版信息
Biotechnol Prog. 2009 Jan-Feb;25(1):147-50. doi: 10.1002/btpr.90.
After illumination with white, linearly polarized light (WLPL), cyclodextrin glycosyltransferase produced mixture of alpha-, beta-, and gamma-cyclodextrins (CD) with higher overall yield than did that enzyme when nonilluminated. The illumination also influenced the ratio of those CD and that effect depended on concentration of enzyme and illumination time. At a high enzyme concentration (0.64 U/cm(3)), regardless the illumination time, formation of beta-CD predominated. The highest yield of beta-CD was afforded after 1 h illumination and 2 h illumination led to a significant increase in the yield of gamma-CD. Three-month storage of enzyme illuminated with WLPL did not reduce its enhanced activity.
在用白色线偏振光（WLPL）照射后，环糊精糖基转移酶产生的α-、β-和γ-环糊精（CD）混合物的总产率高于未照射时该酶产生的总产率。照射还影响了这些环糊精的比例，且该效应取决于酶的浓度和照射时间。在高酶浓度（0.64 U/cm³）下，无论照射时间如何，β-环糊精的形成占主导。照射1小时后β-环糊精的产率最高，照射2小时导致γ-环糊精的产率显著增加。用WLPL照射的酶储存三个月并未降低其增强的活性。
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