Paulo Michele, Salvador Mirian Mendonça, dos Anjos Neto Filho Mário, Montes Marlise Bonetti Agostinho, Franceschini Silvio Antônio, Toloi Maria Regina Torqueti
Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of São Paulo, Ribeirão Preto, Brazil.
Menopause. 2009 May-Jun;16(3):539-44. doi: 10.1097/gme.0b013e31818d6349.
In this study, we determined the protective effect of isoflavones from Glycine max on human umbilical vein endothelial cell (ECV304) damage induced by hydrogen peroxide (H2O2) and on nitric oxide (NO) production.
We studied the regulation of NO synthesis in cultured human endothelial cells by phytoestrogens contained in soy extracts in the presence or absence of ICI 182,780 or N(omega)-nitro-L-arginine methyl esther and determined the protective effect of these isoflavones on ECV304 damage induced by H2O2.
We show that soy extracts activate NO synthesis in endothelial cells and protect against cell damage.
In conclusion, soy isoflavones markedly protect ECV304 cells against H2O2 damage and promote NO synthesizing. Therefore, these isoflavones can potentially act as an NO promoter and as an antioxidant.
在本研究中，我们测定了来自大豆的异黄酮对过氧化氢（H2O2）诱导的人脐静脉内皮细胞（ECV304）损伤的保护作用以及对一氧化氮（NO）生成的影响。
我们研究了在存在或不存在ICI 182,780或N（ω）-硝基-L-精氨酸甲酯的情况下，大豆提取物中含有的植物雌激素对培养的人内皮细胞中NO合成的调节作用，并测定了这些异黄酮对H2O2诱导的ECV304损伤的保护作用。
我们发现大豆提取物可激活内皮细胞中的NO合成并防止细胞损伤。
总之，大豆异黄酮可显著保护ECV304细胞免受H2O2损伤并促进NO合成。因此，这些异黄酮可能潜在地作为NO促进剂和抗氧化剂发挥作用。
