Monteiro Cristina M, Marques Ana P G C, Castro Paula M L, Xavier Malcata F
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072, Porto, Portugal.
Biodegradation. 2009 Sep;20(5):629-41. doi: 10.1007/s10532-009-9250-6. Epub 2009 Feb 19.
Microalgae have been proven efficient biological vectors for heavy metal uptake. In order to further study their biosorption potential, a strain of Desmodesmus pleiomorphus (L) was isolated from a strongly contaminated industrial site in Portugal. Under different initial Zn(2+) concentrations, metal removal by that strain reached a maximum of 360 mg Zn/g biomass after 7 days, at 30 mg Zn/l, after an initial rapid phase of uptake. Comparative studies were carried out using a strain of the same microalgal species that is commercially available (ACOI 561): when exposed to 30 mg Zn/l, it could remove only 81.8 mg Zn/g biomass. Biosorption experiments using inactivated biomass of the isolated strain reached a maximum Zn(2+) uptake of 103.7 mg/g. Metal removal at various initial pH values was studied as well; higher removal was obtained at pH 5.0. The microalga strain L, isolated from the contaminated site, exhibited a much higher removal capacity than the commercial strain, and the living biomass yielded higher levels of metal removal than its inactivated form.
微藻已被证明是用于重金属吸收的高效生物载体。为了进一步研究它们的生物吸附潜力，从葡萄牙一个污染严重的工业场地分离出了一株多形栅藻（Desmodesmus pleiomorphus (L)）。在不同的初始锌离子浓度下，该菌株在初始快速吸收阶段后，7天内对锌的去除量在锌离子浓度为30毫克/升时达到最大值，为360毫克锌/克生物量。使用一种市售的同一种微藻菌株（ACOI 561）进行了对比研究：当暴露于30毫克锌/升时，它只能去除81.8毫克锌/克生物量。使用分离菌株的灭活生物量进行的生物吸附实验中，锌离子的最大吸收量为103.7毫克/克。还研究了在不同初始pH值下的金属去除情况；在pH 5.0时获得了更高的去除率。从污染场地分离出的微藻菌株L表现出比市售菌株高得多的去除能力，并且活生物量比其灭活形式产生更高水平的金属去除量。
