Pérez Silva Rosa María, Abalos Rodríguez Arelis, Gómez Montes De Oca José Manuel, Cantero Moreno Domingo
Study Centre of Industrial Biotechnology, Faculty of Natural Sciences, University of East Santiago of Cuba, CP 90500, Cuba.
Bioresour Technol. 2009 Feb;100(4):1533-8. doi: 10.1016/j.biortech.2008.06.057. Epub 2008 Oct 23.
The study describes the sorption of Cr, Cu, Mn and Zn by Pseudomonas aeruginosa AT18 isolated from a site contaminated with petroleum and heavy metals. The concentrations studied were 50, 49, 60 and 70 (mg L(-1)) for Cr, Cu, Mn and Zn, respectively. The solution pH and ionic strength were very important factors in the metal biosorption performance and the biosorption capacity of P. aeruginosa AT18 for Cr3+,Cu2+, Mn2+ and Zn2+. In aqueous solution, the biosorption increased with increasing pH in the range 5.46-7.72. The results obtained in the experimental assays show that P. aeruginosa AT18 has the capacity for biosorption of the metallic ions Cr3+, Cu2+ and Zn2+ in solutions, although its capacity for the sorption of manganese is low (22.39 mg Mn2+/g of biomass) in comparison to the Cr3+, Cu2+ and Zn2+ ions, as shown by the individual analyses. However, 20% of the manganese was removed from an initial concentration of 49.0 mg L(-1), with a Qm value similar to that obtained in solutions containing mixtures of Cr3+, Cu2+, Mn2+and Zn2+. The chromium level sorbed by P. aeruginosa AT18 biomass was higher than that for Cu, Mn and Zn, with 100% removal in the pH range 7.00-7.72 and a Qm of 121.90-200.00 mg of Cr3+/g of biomass. The removal of Cr, Cu and Zn is also a result of precipitation processes.
该研究描述了从受石油和重金属污染的场地分离出的铜绿假单胞菌AT18对铬、铜、锰和锌的吸附情况。所研究的铬、铜、锰和锌的浓度分别为50、49、60和70（mg L(-1)）。溶液的pH值和离子强度是影响金属生物吸附性能以及铜绿假单胞菌AT18对Cr3+、Cu2+、Mn2+和Zn2+生物吸附能力的非常重要的因素。在水溶液中，生物吸附在5.46 - 7.72的pH范围内随pH值升高而增加。实验分析结果表明，铜绿假单胞菌AT18具有对溶液中金属离子Cr3+、Cu2+和Zn2+的生物吸附能力，不过与Cr3+、Cu2+和Zn2+离子相比，其对锰的吸附能力较低（22.39 mg Mn2+/g生物量）。然而，从初始浓度49.0 mg L(-1)中去除了20%的锰，其Qm值与在含有Cr3+、Cu2+、Mn2+和Zn2+混合物的溶液中获得的值相似。铜绿假单胞菌AT18生物量吸附的铬水平高于铜、锰和锌，在pH值7.00 - 7.72范围内去除率为100%，Qm为121.90 - 200.00 mg Cr3+/g生物量。铬、铜和锌的去除也是沉淀过程的结果。
