EMPA, Swiss Federal Laboratories for Materials Testing and Research, Wood Laboratory, Group of Wood Protection and Biotechnology, Lerchenfeldstrasse 5, St. Gallen, Switzerland.
J Appl Microbiol. 2009 May;106(5):1734-42. doi: 10.1111/j.1365-2672.2008.04138.x. Epub 2009 Feb 16.
To evaluate the influence of water activity (a(w)), temperature and pH on the radial growth and lag phase of Physisporinus vitreus (E-642), a basidiomycete was used in the biotechnological process of bioincising.
Radial growth was monitored for 20 days on malt extract agar medium. Five levels of a(w) (0.998, 0.982, 0.955, 0.928, 0.892) were combined with three incubation temperatures (10, 15, 20 degrees C) and three pH values (4, 5, 6). Data analyses showed a highly significant effect of a(w) and temperature (P < 0.0001) and a significant effect of pH (P < 0.05). The radial growth rate and lag phase of P. vitreus were very sensitive to a(w) reduction. Although P. vitreus was able to grow at all the selected temperatures and pH values, the lag phase increased with decreasing a(w) and growth became inhibited at a(w) = 0.955. Optimal conditions for growth of P. vitreus were a(w) = 0.998, 20 degrees C and pH 5. The response surface model provided reliable estimates of these growth parameters and confirmed a greater dependence on a(w) than on temperature or pH under in vitro conditions.
Low levels of a(w) can prevent growth of P. vitreus, so wood moisture content should be adjusted accordingly.
Implementation of these results should contribute towards the optimization and efficiency of bioincising.
评估水活度（a(w)）、温度和 pH 值对 Physisporinus vitreus（E-642）的径向生长和滞后期的影响，该担子菌用于生物刻蚀的生物技术过程。
在麦芽提取物琼脂培养基上监测 20 天的径向生长。将 5 个 a(w)水平（0.998、0.982、0.955、0.928、0.892）与 3 个培养温度（10、15、20°C）和 3 个 pH 值（4、5、6）相结合。数据分析显示 a(w)和温度（P < 0.0001）有高度显著影响，pH 值（P < 0.05）有显著影响。P. vitreus 的径向生长速率和滞后期对 a(w)的降低非常敏感。尽管 P. vitreus 能够在所有选定的温度和 pH 值下生长，但随着 a(w)的降低，滞后期增加，在 a(w) = 0.955 时生长受到抑制。P. vitreus 生长的最佳条件为 a(w) = 0.998、20°C 和 pH 5。响应面模型提供了这些生长参数的可靠估计，并证实了在体外条件下，P. vitreus 对 a(w)的依赖性大于对温度或 pH 值的依赖性。
低水平的 a(w)可以阻止 P. vitreus 的生长，因此木材的水分含量应相应调整。
实施这些结果应有助于生物刻蚀的优化和效率。
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