Alarcón-Reverte R, Moss S R
Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK.
Commun Agric Appl Biol Sci. 2008;73(4):899-902.
Resistance to five ACCase-inhibiting herbicides (dictofop, fluazifop, tralkoxydim, cycloxydim and pinoxaden) was investigated in 55 Lolium multiflorum populations from 50 farms in England. Resistance screening experiments at whole plant level showed differences in the response to each herbicide suggesting different resistance mechanisms for different herbicides, despite all of them having the same mode of action. Plants from populations resistant to at least one herbicide were analysed at the molecular level using the derived Cleaved Amplified Polymorphic Sequence method (dCAPS). Three single-site point mutations (ile-1781-leu, asp-2078-gly and cys-2088-arg) known to confer resistance to ACCase inhibitors were found in 13 populations. In the remaining resistant populations a more specific mutation or an enhanced metabolism mechanism might be involved.
对来自英格兰50个农场的55个多花黑麦草种群进行了对五种乙酰辅酶A羧化酶（ACCase）抑制性除草剂（禾草灵、精稳杀得、唑啉草酯、噻草酮和丙酯草醚）的抗性研究。在整株水平上进行的抗性筛选实验表明，对每种除草剂的反应存在差异，这表明尽管所有除草剂具有相同的作用模式，但不同除草剂的抗性机制不同。使用衍生的酶切扩增多态性序列（dCAPS）方法在分子水平上分析了对至少一种除草剂具有抗性的种群中的植物。在13个种群中发现了已知赋予对ACCase抑制剂抗性的三个单一位点突变（异亮氨酸-1781-亮氨酸、天冬氨酸-2078-甘氨酸和半胱氨酸-2088-精氨酸）。在其余的抗性种群中，可能涉及更特异的突变或增强的代谢机制。
