Yang W H, Zhang W C, Lu X M, Jiang G S, Gao P J
State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong, P.R. China.
Lett Appl Microbiol. 2009 Apr;48(4):393-7. doi: 10.1111/j.1472-765X.2008.02508.x. Epub 2009 Feb 18.
To isolate a novel antibiotic termed AF from fermentation broth of Penicillium sp. M03 and to examine its antimicrobial activity, biological properties and structure characteristics.
Sephadex LH-20 and HPLC were used to purify AF from fermentation broth of Penicillium sp. M03. The antimicrobial activity of AF was evaluated with the agar diffusion test. Amino acid and monosaccharide composition of AF was analysed by a HITACHI 835 detector and HPLC assay, respectively. Matrix-assisted laser desorption time of flight mass spectrometry, FT-IR and (1)H nuclear magnetic resonance spectra analyses were performed to examine the initial structure of AF. Eighty milligrams of AF was isolated as white powder from 1-l Penicillium sp. M03 fermentation broth. It consists of five amino acid and two monosaccharide residues and the molecular weight of it was 1017, and it was stable to beta-lactamase, heat, acid and alkali. AF showed inhibitory activity to a wide range of bacteria, particularly to multidrug-resistant Staphylococcus aureus.
AF was a novel antibacterial glycopeptide with a broad inhibitory spectrum to pathogenic bacteria including multidrug-resistant agents. Furthermore, it is difficult to generate bacteria resistant to AF.
Characterization of AF made it a potential antibiotic to fight against antibiotic-resistant bacterial pathogens.
从青霉菌株M03的发酵液中分离一种名为AF的新型抗生素，并检测其抗菌活性、生物学特性和结构特征。
采用葡聚糖凝胶LH - 20和高效液相色谱法从青霉菌株M03的发酵液中纯化AF。通过琼脂扩散试验评估AF的抗菌活性。分别用日立835检测器和高效液相色谱法分析AF的氨基酸和单糖组成。进行基质辅助激光解吸飞行时间质谱、傅里叶变换红外光谱和氢核磁共振光谱分析以检测AF的初始结构。从1升青霉菌株M03发酵液中分离出80毫克白色粉末状的AF。它由五个氨基酸和两个单糖残基组成，分子量为1017，对β-内酰胺酶、热、酸和碱稳定。AF对多种细菌具有抑制活性，尤其对多重耐药金黄色葡萄球菌。
AF是一种新型抗菌糖肽，对包括多重耐药菌在内的病原菌具有广泛的抑制谱。此外，细菌很难对AF产生耐药性。
AF的特性使其成为对抗耐药性细菌病原体的潜在抗生素。
