Karnati Srikanth, Baumgart-Vogt Eveline
Division of Medical Cell Biology, Institute for Anatomy and Cell Biology II, Justus Liebig University, Aulweg 123, 35385 Giessen, Germany.
Histochem Cell Biol. 2009 Apr;131(4):447-54. doi: 10.1007/s00418-009-0566-4. Epub 2009 Feb 20.
Peroxisomes are intimately involved in the metabolism of reactive oxygen species, in the synthesis of ether lipids and of polyunsaturated fatty acids as well as in the beta-oxidation of bioactive and toxic lipid derivatives. Therefore, the metabolic pathways of this organelle might play an important role in pulmonary biology by protection of inner pulmonary surface epithelia against oxidative stress, induced by the high oxygen levels in the air and/or by regulation of the lipid homeostasis in pulmonary epithelia and the pulmonary surfactant film. In this article, original results on the distribution of peroxisomal marker proteins, involved in the biogenesis, ROS- and lipid-metabolism of this organelle in the bronchiolar epithelium and the alveolar region of the adult human lung in comparison to newborn and adult murine lungs are presented. In addition, we investigated the expression of the PEX11beta-mRNA, encoding a protein involved in peroxisomal division. Our study revealed significant differences in the abundance and distribution of peroxisomal proteins in distinct cell types of the lung and different developmental stages and led to the discovery of species-specific differences in the peroxisomal compartment in pulmonary epithelia between mouse and man. Finally, the structure and general biology of pulmonary airways-with special emphasis on Clara cells-are reviewed and discussed in relation to peroxisomal metabolism and proliferation. Future prospects of peroxisomes and Pex11 proteins for pulmonary cell biology are highlighted.
过氧化物酶体密切参与活性氧的代谢、醚脂和多不饱和脂肪酸的合成，以及生物活性和有毒脂质衍生物的β氧化。因此，该细胞器的代谢途径可能通过保护肺内表面上皮免受空气中高氧水平诱导的氧化应激，和/或通过调节肺上皮细胞和肺表面活性物质膜中的脂质稳态，在肺生物学中发挥重要作用。在本文中，展示了与新生和成年小鼠肺相比，参与该细胞器在成人肺细支气管上皮和肺泡区域的生物发生、活性氧和脂质代谢的过氧化物酶体标记蛋白分布的原始结果。此外，我们研究了编码参与过氧化物酶体分裂的蛋白质的PEX11β - mRNA的表达。我们的研究揭示了肺中不同细胞类型以及不同发育阶段过氧化物酶体蛋白丰度和分布的显著差异，并发现了小鼠和人肺上皮细胞过氧化物酶体区室的物种特异性差异。最后，结合过氧化物酶体代谢和增殖对肺气道的结构和一般生物学——特别强调克拉拉细胞——进行了综述和讨论。强调了过氧化物酶体和Pex11蛋白在肺细胞生物学中的未来前景。
