Department of Otorhinolaryngology, Head and Neck Surgery, University of Giessen, Klinikstrasse 33, Ebene-1, D-35392 Gießen, Germany.
Institute of Pathology, Justus Liebig University, Langhansstrasse 10, D-35392 Gießen, Germany.
Int J Mol Sci. 2021 Jul 23;22(15):7872. doi: 10.3390/ijms22157872.
Salivary gland cancers are rare but aggressive tumors that have poor prognosis and lack effective cure. Of those, parotid tumors constitute the majority. Functioning as metabolic machinery contributing to cellular redox balance, peroxisomes have emerged as crucial players in tumorigenesis. Studies on murine and human cells have examined the role of peroxisomes in carcinogenesis with conflicting results. These studies either examined the consequences of altered peroxisomal proliferators or compared their expression in healthy and neoplastic tissues. None, however, examined such differences exclusively in human parotid tissue or extended comparison to peroxisomal proteins and their associated gene expressions. Therefore, we examined differences in peroxisomal dynamics in parotid tumors of different morphologies. Using immunofluorescence and quantitative PCR, we compared the expression levels of key peroxisomal enzymes and proliferators in healthy and neoplastic parotid tissue samples. Three parotid tumor subtypes were examined: pleomorphic adenoma, mucoepidermoid carcinoma and acinic cell carcinoma. We observed higher expression of peroxisomal matrix proteins in neoplastic samples with exceptional down regulation of certain enzymes; however, the degree of expression varied between tumor subtypes. Our findings confirm previous experimental results on other organ tissues and suggest peroxisomes as possible therapeutic targets or markers in all or certain subtypes of parotid neoplasms.
唾液腺癌是罕见但侵袭性强的肿瘤，预后不良，缺乏有效治疗方法。其中，腮腺肿瘤占多数。过氧化物酶体作为代谢机制的一部分，有助于细胞氧化还原平衡，现已成为肿瘤发生的关键因素。对鼠类和人类细胞的研究已经研究了过氧化物酶体在致癌作用中的作用，但结果存在冲突。这些研究要么检查了过氧化物酶体增殖剂改变的后果，要么比较了它们在健康和肿瘤组织中的表达。然而，没有一项研究专门在人类腮腺组织中检查这些差异，也没有将其与过氧化物酶体蛋白及其相关基因表达进行扩展比较。因此，我们检查了不同形态的腮腺肿瘤中过氧化物酶体动力学的差异。我们使用免疫荧光和定量 PCR 比较了健康和肿瘤腮腺组织样本中关键过氧化物酶体酶和增殖剂的表达水平。研究了三种腮腺肿瘤亚型：多形性腺瘤、黏液表皮样癌和腺泡细胞癌。我们观察到在肿瘤样本中过氧化物酶体基质蛋白的表达水平更高，某些酶的表达水平异常下调；然而，在肿瘤亚型之间，表达程度有所不同。我们的研究结果证实了其他器官组织的先前实验结果，并表明过氧化物酶体可能是所有或某些腮腺肿瘤亚型的治疗靶点或标志物。
