Manicheva O A, Lasunskaia E B, Zhuravlev V Iu, Otten T F, Barnaulov A O, Mokrousov I V, Pavlova M V, Vishnevskiĭ B I, Narvskaia O V
Probl Tuberk Bolezn Legk. 2008(12):18-22.
The authors studied drug sensitivity, mutations in the katG, in-hA, alpC, rpoB genes, virulence via the cytotoxicity test on THP-1 cells, and the viability and genetic affiliation of 53 clinical M. tuberculosis isolates versus data on the form and dynamics of a process. Sensitive and resistant strains did not significantly differ in viability and cytotoxicity. The highest death of infected macrophages was observed was seen with infection of M. tuberculosis of the Beijing B0 genotype, the least one seen with that of LAM with the similar rate of multiple drug resistance. There was a correlation of the changes in the count of lymphocytes in patients with the genetic affiliation of a causative agent. The severest course of the tuberculous process was observed in baseline lymphopenia (before treatment) in combination with multidrug resistance of mycobacteria, high and moderate cytotoxicity and high viability. Ser-Leu 531 mutation resulted in cross resistance to rifampicin and mycobutin in most cases.
作者研究了53株临床结核分枝杆菌分离株的药物敏感性、katG、inhA、alpC、rpoB基因的突变情况，通过对THP-1细胞进行细胞毒性试验研究其毒力，以及其生存能力和基因归属，并与疾病过程的形式和动态数据进行对比。敏感菌株和耐药菌株在生存能力和细胞毒性方面无显著差异。感染北京B0基因型结核分枝杆菌时，观察到被感染巨噬细胞的死亡率最高；感染具有相似多重耐药率的LAM基因型结核分枝杆菌时，死亡率最低。病原体的基因归属与患者淋巴细胞计数的变化存在相关性。在基线淋巴细胞减少（治疗前）合并分枝杆菌多重耐药、高和中度细胞毒性以及高生存能力的情况下，观察到结核病程最为严重。在大多数情况下，Ser-Leu 531突变导致对利福平和结核霉素产生交叉耐药。
