Division of Reproductive Medicine, Department of Perinatal and Maternal Care, National Center for Child Health and Development, Tokyo 157-8535, Japan.
Fertil Steril. 2010 May 1;93(7):2405-10. doi: 10.1016/j.fertnstert.2009.01.063. Epub 2009 Feb 20.
To evaluate the efficacy of a nylon mesh container in vitrification of human embryos and to determine the optimal osmotic pressure of the initial thawing solution.
Retrospective analysis.
National Center for Child Health and Development, Tokyo, Japan.
PATIENT(S): Infertile patients undergoing either in vitro fertilization or intracytoplasmic sperm injection in our hospital.
INTERVENTION(S): Embryos, at the cleavage stage, were cryopreserved using the vitrification method in either a plastic straw or a nylon mesh container. The embryos were thawed using an initial osmotic pressure of either 0.5 M or 1.0 M sucrose with subsequent step-wise dilution. After thawing, the embryos were transferred to the uterus.
MAIN OUTCOME MEASURE(S): Survival rate of blastomeres, embryo survival rate, implantation, and pregnancy rates, cancellation rate because of embryo damage.
RESULT(S): Use of nylon mesh and the 1.0 M sucrose thawing solution significantly improved blastomere survival rate (98.0 +/- 1.0%, mean +/- SEM), pregnancy rate (41.0%) and implantation rate (32.3%).
CONCLUSION(S): Vitrification using a nylon mesh container and subsequent thawing in a 1.0 M sucrose solution is an easy and inexpensive method that improves the reliability of embryo cryopreservation of embryos without adverse effects on clinical outcomes.
评估尼龙网容器在人类胚胎玻璃化冷冻中的效果，并确定初始解冻溶液的最佳渗透压。
回顾性分析。
日本东京国立儿童健康与发展中心。
在我院接受体外受精或卵胞浆内单精子注射的不孕患者。
使用玻璃化冷冻方法，将卵裂期胚胎分别保存在塑料吸管或尼龙网容器中。胚胎使用初始渗透压为 0.5 M 或 1.0 M 蔗糖的溶液进行解冻，随后进行逐步稀释。解冻后，胚胎被移植到子宫内。
卵裂球存活率、胚胎存活率、着床率和妊娠率、因胚胎损伤而取消率。
使用尼龙网和 1.0 M 蔗糖解冻溶液显著提高了卵裂球存活率（98.0 +/- 1.0%，平均值 +/- SEM）、妊娠率（41.0%）和着床率（32.3%）。
使用尼龙网容器进行玻璃化冷冻，随后在 1.0 M 蔗糖溶液中解冻，是一种简单且经济实惠的方法，可提高胚胎冷冻的可靠性，而对临床结局无不良影响。
