Lei Lei, Guan Na, Xu Yan-Ning, Zhang Qing-Hua, Shen Jing-Ling, Jin Lian-Hong
Department of Histology, Harbin Medical University, 194 Xuefu Road, Nangang District, Harbin 150081, China.
Zygote. 2009 May;17(2):125-30. doi: 10.1017/S0967199409005206. Epub 2009 Feb 23.
Polyploid mouse embryos are important models for understanding the mechanisms of cleavage and preimplantation development in mammals. In this study, hexaploid (6n) mouse embryos were produced by the electrofusion of blastomeres from diploid (2n) and tetraploid (4n) embryos at the 2-cell stage. Furthermore, the developmental pattern of hexaploid embryos was evaluated by blastocyst rate, cell number, karyotype analysis, cytoskeleton staining and Oct-4 immunofluorescence. The results showed that 72.7% of the hexaploid embryos were able to develop to the blastocyst stage, which is a lower number than that found with normal diploid embryos (98.0%, p < 0.05). The cell number in hexaploid blastocyst was 12.3 +/- 2.0, which was less than that found in diploid or tetraploid blastocysts (41.2 +/- 7.2; 18.4 +/- 3.5). Karyotype analysis confirmed that the number of chromosomes in hexaploid embryos was 120. beta-Tubulin and Oct-4 immunofluorescence indicated that the hexaploid blastocysts were nearly lacking inner cell mass (ICM), but some blastomeres did show Oct-4-positive expression.
多倍体小鼠胚胎是理解哺乳动物卵裂和植入前发育机制的重要模型。在本研究中，通过将二倍体（2n）和四倍体（4n）胚胎的卵裂球在2细胞阶段进行电融合，产生了六倍体（6n）小鼠胚胎。此外，通过囊胚率、细胞数量、核型分析、细胞骨架染色和Oct-4免疫荧光对六倍体胚胎的发育模式进行了评估。结果显示，72.7%的六倍体胚胎能够发育到囊胚阶段，这一数量低于正常二倍体胚胎（98.0%，p<0.05）。六倍体囊胚中的细胞数量为12.3±2.0，低于二倍体或四倍体囊胚中的细胞数量（41.2±7.2；18.4±3.5）。核型分析证实六倍体胚胎中的染色体数量为120条。β-微管蛋白和Oct-4免疫荧光表明，六倍体囊胚几乎缺乏内细胞团（ICM），但一些卵裂球确实显示出Oct-4阳性表达。
