Terrace John D, Hay David C, Samuel Kay, Payne Catherine, Anderson Richard A, Currie Ian S, Parks Rowan W, Forbes Stuart J, Ross James A
Centre for Regenerative Medicine, University of Edinburgh Medical School, Chancellor's Building, 49 Little France Crescent, EH16 4SB, Edinburgh, UK.
Exp Cell Res. 2009 Aug 1;315(13):2141-53. doi: 10.1016/j.yexcr.2009.02.004. Epub 2009 Feb 20.
Side population (SP) cells have recently been identified in a number of tissues although their phenotype and functional abilities are poorly understood. Surface marker characterisation and functional assessment of developing liver SP cells might allow for their isolation and manipulation using clinically relevant techniques. It was hypothesised that SP cells are present early during human liver development and contribute to haematopoietic and epithelial lineage generation. Whilst the SP population remained positive for CD34 during the 1st and 2nd trimester, 1st trimester SP cells were more highly enriched for haematopoietic and epithelial progenitor activity than those from the 2nd trimester in vitro. Marker expression and functional similarities indicate that SP cells in developing human liver may share a temporal relationship with oval/progenitor cells, responsible for liver regeneration after massive or chronic hepatic injury. Furthermore, modification of SP integrin expression during development suggests a potential adaptive interaction with niche components such as fibronectin. Improved understanding of developing human liver SP cells will contribute to the generation of novel cell-based therapies for liver disease.
尽管对侧群（SP）细胞的表型和功能能力了解甚少，但最近已在多种组织中鉴定出该细胞。对发育中的肝脏SP细胞进行表面标志物表征和功能评估，可能有助于使用临床相关技术对其进行分离和操控。研究假设，SP细胞在人类肝脏发育早期即已存在，并有助于造血和上皮谱系的生成。虽然在妊娠早期和中期，SP群体对CD34保持阳性，但在体外，妊娠早期的SP细胞比妊娠中期的SP细胞在造血和上皮祖细胞活性方面富集程度更高。标志物表达和功能相似性表明，发育中的人类肝脏中的SP细胞可能与卵圆细胞/祖细胞存在时间上的关系，后者负责在大规模或慢性肝损伤后进行肝脏再生。此外，发育过程中SP整合素表达的改变表明其与诸如纤连蛋白等生态位成分存在潜在的适应性相互作用。对发育中的人类肝脏SP细胞的深入了解将有助于开发针对肝脏疾病的新型细胞疗法。
