Shapot V S, Gorozhanskaia E G, Liubimova N A
Biokhimiia. 1976 Oct;41(10):1766-72.
The total activity of hexokinase (HK) and HK isoenzymic spectrum of the normal liver and slowly groming hepatoma 49 did not show any essential differences. However, the HK total activity and the relative and absolute contents of isoenzyme HK-3 were increased in hepatomas 61 and especially in the rapidly growing hepatoma 22-a. The glucokinase activity decreases in the hepatiomas 49 and 61 and disappears in the rapidly growing hepatoma 22-a. The glucose content in hepatoma 49 was slightly lower than in the normal liver, whereas in other hepatoma no traces of glucose could be detected. At low glucose concentration in the medium (0,1 mM), i.e. under conditions simulating those characteristic of tumors in vivo, the predominant form of HK in all hepatomas studied was found to be HK-3. In the liver of hepatoma-bearing mice some shifts in the value of total HK activity and its isoenzymic spectrum, reminding one of those found in hepatomas themselves, were observed. Unequal deviations in the total HK activity and its isoenzymic spectrum in hepatomas with different degrees of malignancy indicate that these characteristics are secondary rather than primary events depending on tumour progression.
正常肝脏和生长缓慢的肝癌49的己糖激酶（HK）总活性及HK同工酶谱未显示出任何本质差异。然而，肝癌61尤其是快速生长的肝癌22-a中HK总活性以及同工酶HK-3的相对和绝对含量均有所增加。肝癌49和61中的葡萄糖激酶活性降低，而在快速生长的肝癌22-a中则消失。肝癌49中的葡萄糖含量略低于正常肝脏，而在其他肝癌中未检测到葡萄糖痕迹。在培养基中葡萄糖浓度较低（0.1 mM）时，即在模拟体内肿瘤特征的条件下，在所研究的所有肝癌中，HK的主要形式均为HK-3。在荷瘤小鼠的肝脏中，观察到总HK活性值及其同工酶谱出现了一些变化，类似于在肝癌本身中发现的变化。不同恶性程度的肝癌中HK总活性及其同工酶谱的偏差不等，表明这些特征是继发而非取决于肿瘤进展的原发事件。
