丙型肝炎病毒感染患者中HPA-1至5人类血小板抗原的等位基因频率。

Allelic frequencies of HPA-1 to 5 human platelet antigens in patients infected with hepatitis C virus.

作者信息

Verdichio-Moraes Camila Fernanda, Toralles-Pereira Cecília, Grotto Rejane Maria Tommasini, Silva Giovanni Faria, Pardini Maria Inês de Moura Campos

机构信息

Molecular Biology Laboratory of Blood Transfusion Center, Botucatu Medical School, São Paulo State University, UNESP, Botucatu-SP, Brazil.

出版信息

J Med Virol. 2009 Apr;81(4):757-9. doi: 10.1002/jmv.21411.

DOI:10.1002/jmv.21411

PMID:19235843

Abstract

Studies have suggested that hepatitis C virus (HCV) may infect not only hepatocytes but may also be carried by platelets. Platelets express more than 20 polymorphic antigenic determinants on their surface, which are called human platelet antigens (HPA). To determine the allele frequency of the HPA-1 to -5 in patients infected with HCV, blood samples were collected from 257 blood donors for the control group and from 191 patients infected with HCV. DNA was isolated and amplified for genes HPA-1 to -4 using PCR Sequence Specific Primers (PCR-SSP) and HPA-5 using PCR-Restriction Fragment Length Polymorphism (PCR-RFLP). The allelic and genotypic frequency of HPA-5a in patients infected with HCV was found to be significantly lower (P < 0.05) than in the controls, and HPA-5b from patients infected with HCV was significantly higher (P < 0.05) than in controls. The increase in HPA-5b allelic frequency in HCV infection may indicate a possible association between HCV infection and HPAs.

摘要

研究表明，丙型肝炎病毒（HCV）不仅可能感染肝细胞，还可能由血小板携带。血小板在其表面表达20多种多态性抗原决定簇，这些被称为人类血小板抗原（HPA）。为了确定丙型肝炎病毒感染患者中HPA - 1至 - 5的等位基因频率，从257名献血者中采集血样作为对照组，从191名丙型肝炎病毒感染患者中采集血样。使用PCR序列特异性引物（PCR - SSP）分离并扩增HPA - 1至 - 4的基因，使用PCR - 限制性片段长度多态性（PCR - RFLP）扩增HPA - 5的基因。发现丙型肝炎病毒感染患者中HPA - 5a的等位基因和基因型频率显著低于对照组（P < 0.05），而丙型肝炎病毒感染患者的HPA - 5b显著高于对照组（P < 0.05）。丙型肝炎病毒感染中HPA - 5b等位基因频率的增加可能表明丙型肝炎病毒感染与HPA之间存在可能的关联。