Loeser Eric, Babiak Stanislaw, Drumm Patrick
Pharmaceutical and Analytical Development, Novartis Pharmaceuticals, East Hanover, NJ, USA.
J Chromatogr A. 2009 Apr 10;1216(15):3409-12. doi: 10.1016/j.chroma.2009.02.012. Epub 2009 Feb 11.
It has recently been shown that the use of strong organic solvents as diluent is possible in RPLC, provided that the solvent used as diluent is retained more strongly by the column than the analytes in the sample. In this study, the phenomenon was further studied experimentally using several water-immiscible solvents (ethyl acetate, isopropyl acetate, and methyl isobutyl ketone) and several model analyte compounds. In all cases, analyte peak distortion was minimal provided the analyte eluted earlier than the diluting solvent, in contrast to substantial broadening and distortion when the analyte eluted after the diluting solvent. The potential analytical utility of this approach is discussed, and an example of a practical application is also presented.
最近研究表明，在反相液相色谱法（RPLC）中使用强有机溶剂作为稀释剂是可行的，前提是用作稀释剂的溶剂在色谱柱上的保留比样品中的分析物更强。在本研究中，使用几种与水不混溶的溶剂（乙酸乙酯、乙酸异丙酯和甲基异丁基酮）和几种模型分析物化合物对该现象进行了进一步的实验研究。在所有情况下，只要分析物比稀释剂溶剂洗脱得早，分析物峰的畸变就最小，相反，当分析物在稀释剂溶剂之后洗脱时，会出现明显的展宽和畸变。讨论了这种方法潜在的分析用途，并给出了一个实际应用的例子。
