Gallie D R, Feder J N, Schimke R T, Walbot V
Department of Biological Sciences, Stanford University, CA 94305-5020.
Nucleic Acids Res. 1991 Sep 25;19(18):5031-6. doi: 10.1093/nar/19.18.5031.
The 3'-untranslated region (UTR) of tobacco mosaic virus (TMV), which terminates in a tRNA-like structure, functionally substitutes for a poly(A) tail in both plant and animal cells. The addition of the TMV 3'-UTR to chimeric mRNA constructs increases their expression up to 100-fold, increasing both translational efficiency and mRNA stability. The domain largely responsible for the regulation maps to a 72 base region immediately upstream of the tRNA-like structure, however, the 3'-terminal, tRNA-like structure is required for full function. Its contribution is lost if separated from the upstream pseudoknot domain by as few as 5 bases or if 6 bases are removed from the 3'-terminus. Sequence addition to the 3'-terminus of the TMV 3'UTR or the upstream pseudoknot domain inhibits function in both tobacco and Chinese hamster ovary cells.
烟草花叶病毒（TMV）的3'非翻译区（UTR）以类似tRNA的结构结尾，在植物和动物细胞中都能在功能上替代poly(A)尾。将TMV 3'-UTR添加到嵌合mRNA构建体中可使其表达增加高达100倍，同时提高翻译效率和mRNA稳定性。在很大程度上负责这种调控的结构域定位在类似tRNA结构上游紧邻的一个72个碱基的区域，然而，3'末端类似tRNA的结构对于其完整功能是必需的。如果与上游假结结构域相隔仅5个碱基，或者从3'末端去除6个碱基，其作用就会丧失。在TMV 3'UTR的3'末端或上游假结结构域添加序列会抑制烟草和中国仓鼠卵巢细胞中的功能。
