烟草花叶病毒类tRNA结构在细胞质基因调控中的功能分析
Functional analysis of the tobacco mosaic virus tRNA-like structure in cytoplasmic gene regulation.
作者信息
Gallie D R, Feder J N, Schimke R T, Walbot V
机构信息
Department of Biological Sciences, Stanford University, CA 94305-5020.
出版信息
Nucleic Acids Res. 1991 Sep 25;19(18):5031-6. doi: 10.1093/nar/19.18.5031.
Abstract
The 3'-untranslated region (UTR) of tobacco mosaic virus (TMV), which terminates in a tRNA-like structure, functionally substitutes for a poly(A) tail in both plant and animal cells. The addition of the TMV 3'-UTR to chimeric mRNA constructs increases their expression up to 100-fold, increasing both translational efficiency and mRNA stability. The domain largely responsible for the regulation maps to a 72 base region immediately upstream of the tRNA-like structure, however, the 3'-terminal, tRNA-like structure is required for full function. Its contribution is lost if separated from the upstream pseudoknot domain by as few as 5 bases or if 6 bases are removed from the 3'-terminus. Sequence addition to the 3'-terminus of the TMV 3'UTR or the upstream pseudoknot domain inhibits function in both tobacco and Chinese hamster ovary cells.
摘要
烟草花叶病毒（TMV）的3'非翻译区（UTR）以类似tRNA的结构结尾，在植物和动物细胞中都能在功能上替代poly(A)尾。将TMV 3'-UTR添加到嵌合mRNA构建体中可使其表达增加高达100倍，同时提高翻译效率和mRNA稳定性。在很大程度上负责这种调控的结构域定位在类似tRNA结构上游紧邻的一个72个碱基的区域，然而，3'末端类似tRNA的结构对于其完整功能是必需的。如果与上游假结结构域相隔仅5个碱基，或者从3'末端去除6个碱基，其作用就会丧失。在TMV 3'UTR的3'末端或上游假结结构域添加序列会抑制烟草和中国仓鼠卵巢细胞中的功能。
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本文引用的文献
1
TYMV RNA As a substrate of the tRNA nucleotidyltransferase.芜菁黄花叶病毒核糖核酸作为转运核糖核酸核苷酸转移酶的一种底物。
FEBS Lett. 1970 Dec 18;11(5):316-319. doi: 10.1016/0014-5793(70)80557-9.
2
tRNA-like structures in the genomes of RNA viruses.RNA病毒基因组中的类tRNA结构
Prog Nucleic Acid Res Mol Biol. 1982;27:85-104. doi: 10.1016/s0079-6603(08)60598-x.
3
The operon that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12.在大肠杆菌K12中，编码RNA聚合酶σ亚基的操纵子还编码核糖体蛋白S21和DNA引发酶。
Cell. 1983 Feb;32(2):335-49. doi: 10.1016/0092-8674(83)90453-1.
4
The Role of the poly(A) sequence in mammalian messenger RNA.聚腺苷酸序列在哺乳动物信使核糖核酸中的作用。
CRC Crit Rev Biochem. 1981;10(1):1-38. doi: 10.3109/10409238109114634.
5
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.从含有噬菌体SP6启动子的质粒中高效体外合成生物活性RNA和RNA杂交探针。
Nucleic Acids Res. 1984 Sep 25;12(18):7035-56. doi: 10.1093/nar/12.18.7035.
6
Valine-specific tRNA-like structure in turnip yellow mosaic virus RNA.芜菁黄花叶病毒RNA中的缬氨酸特异性类tRNA结构
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1345-52. doi: 10.1073/pnas.67.3.1345.
7
Further studies on the enzymatic aminoacylation of TMV-RNA by histidine.关于组氨酸对烟草花叶病毒核糖核酸（TMV-RNA）进行酶促氨酰化作用的进一步研究。
FEBS Lett. 1974 Jan 15;38(3):287-91. doi: 10.1016/0014-5793(74)80074-8.
8
Elongation factor-viral genome interaction dependent on the aminoacylation of TYMV and TMV RNAs.依赖芜菁黄花叶病毒（TYMV）和烟草花叶病毒（TMV）RNA氨酰化作用的延伸因子-病毒基因组相互作用。
Nat New Biol. 1973 Jan 17;241(107):88-90. doi: 10.1038/newbio241088a0.
9
Binding of histidine to tobacco mosaic virus RNA.组氨酸与烟草花叶病毒核糖核酸的结合
Biochem Biophys Res Commun. 1972 Aug 21;48(4):927-32. doi: 10.1016/0006-291x(72)90697-3.
10
Five pseudoknots are present at the 204 nucleotides long 3' noncoding region of tobacco mosaic virus RNA.在烟草花叶病毒RNA 204个核苷酸长的3'非编码区存在5个假结。
Nucleic Acids Res. 1985 Nov 11;13(21):7673-86. doi: 10.1093/nar/13.21.7673.
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