过度循环会将PCR产物转化为随机长度的高分子量片段。 - Suppr

Suppr

超能文献

Excessive cycling converts PCR products to random-length higher molecular weight fragments.

作者信息

Bell D A, DeMarini D M

机构信息

Genetic Toxicology Division, US Environmental Protection Agency, RTP, NC 27711.

出版信息

Nucleic Acids Res. 1991 Sep 25;19(18):5079. doi: 10.1093/nar/19.18.5079.

DOI:10.1093/nar/19.18.5079

PMID:1923779

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328818/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7a1/328818/be0a337c511b/nar00098-0263-a.jpg

相似文献

Excessive cycling converts PCR products to random-length higher molecular weight fragments.过度循环会将PCR产物转化为随机长度的高分子量片段。

Nucleic Acids Res. 1991 Sep 25;19(18):5079. doi: 10.1093/nar/19.18.5079.

PCR amplification of ribosomal DNA spacer regions of Salmonella enteritidis isolates.

Indian J Exp Biol. 1997 Jun;35(6):668-9.

Amplification of Salmonella chromosomal DNA using the polymerase chain reaction.利用聚合酶链反应扩增沙门氏菌染色体DNA。

Avian Dis. 1994 Jan-Mar;38(1):119-26.

Use of PCR-Ribotyping for the characterisation of Salmonella.

Boll Soc Ital Biol Sper. 1994 Jan-Feb;70(1-2):27-33.

Use of repetitive element palindromic PCR (rep-PCR) for the epidemiologic discrimination of foodborne pathogens.利用重复元件回文PCR（rep-PCR）对食源性病原体进行流行病学鉴别。

Methods Mol Biol. 2009;551:49-58. doi: 10.1007/978-1-60327-999-4_5.

[Optimization of the REP-PCR molecular classification method of Salmonella and its application in drug-resistant strains].[沙门氏菌REP-PCR分子分型方法的优化及其在耐药菌株中的应用]

Wei Sheng Yan Jiu. 2009 Nov;38(6):736-9.

Rapid and sensitive method for detection of Salmonella strains using a combination of polymerase chain reaction and reverse dot-blot hybridization.

FEMS Microbiol Lett. 1993 Dec 1;114(2):167-72. doi: 10.1111/j.1574-6968.1993.tb06568.x.

[Phylogenetic analysis and PCR-restriction fragment length polymophism identification of Salmonella based on grOEL gene sequence].基于groEL基因序列的沙门氏菌系统发育分析及PCR-限制性片段长度多态性鉴定

Nan Fang Yi Ke Da Xue Xue Bao. 2009 Oct;29(10):2037-9, 2043.

[Optimization and primary application of one molecular typing method of Salmonella, enterobacteia repetitive intergenic consensus sequences-based PCR in Salmonella].[沙门氏菌一种分子分型方法的优化及初步应用，基于肠杆菌重复基因间共有序列的聚合酶链反应在沙门氏菌中的应用]

Wei Sheng Yan Jiu. 2008 Sep;37(5):609-12.

Comparison of pulsed field gel electrophoresis and repetitive sequence polymerase chain reaction as genotyping methods for detection of genetic diversity and inferring transmission of Salmonella.脉冲场凝胶电泳和重复序列聚合酶链反应作为检测沙门氏菌遗传多样性和推断传播的基因分型方法的比较

Vet Microbiol. 2004 Jun 3;100(3-4):205-17. doi: 10.1016/j.vetmic.2004.02.009.

引用本文的文献

Detection of (Coleoptera: Curculionidae: Curculioninae) in spp. (Oleaceae) Using Newly Developed PCR Primers.使用新开发的PCR引物检测木蠹象属（鞘翅目：象甲科：象甲亚科）在木犀科植物中的存在情况

Insects. 2024 Apr 30;15(5):320. doi: 10.3390/insects15050320.

Rapid quantitative PCR equipment using photothermal conversion of Au nanoshell.采用金纳米壳光热转换的快速定量 PCR 设备。

Sci Rep. 2024 Feb 16;14(1):3895. doi: 10.1038/s41598-024-54406-0.

Comparison of Different Fixation Methods for Combined Histological and Biomolecular Analysis of Fixed and Decalcified Bone Samples.固定及脱钙骨样本组织学与生物分子联合分析中不同固定方法的比较

Methods Protoc. 2022 Jul 21;5(4):64. doi: 10.3390/mps5040064.

Gene Assembly from Chip-Synthesized Oligonucleotides.基于芯片合成寡核苷酸的基因组装

Curr Protoc Chem Biol. 2012 Mar 1;2012. doi: 10.1002/9780470559277.ch110190.

The use of random amplified polymorphic DNA markers in wheat.利用随机扩增多态性 DNA 标记进行小麦研究。

Theor Appl Genet. 1992 Aug;84(5-6):567-72. doi: 10.1007/BF00224153.

DNA fingerprinting of Peronospora parasitica, a biotrophic fungal pathogen of crucifers.寄生霜霉，十字花科植物生物寄生真菌的 DNA 指纹图谱。

Theor Appl Genet. 1994 Jun;88(3-4):490-6. doi: 10.1007/BF00223666.

Random-amplified-polymorphic DNA markers in sorghum.高粱中的随机扩增多态性 DNA 标记。

Theor Appl Genet. 1994 Sep;89(1):80-8. doi: 10.1007/BF00226987.

Experimental analysis of gene assembly with TopDown one-step real-time gene synthesis.采用自上而下一步实时基因合成法进行基因组装的实验分析。

Nucleic Acids Res. 2009 Apr;37(7):e51. doi: 10.1093/nar/gkp118. Epub 2009 Mar 5.

Factors affecting reliability and reproducibility of amplification-based DNA fingerprinting of representative bacterial pathogens.影响代表性细菌病原体基于扩增的DNA指纹图谱可靠性和可重复性的因素。

J Clin Microbiol. 1997 Feb;35(2):339-46. doi: 10.1128/jcm.35.2.339-346.1997.

Validation of use of whole-cell repetitive extragenic palindromic sequence-based PCR (REP-PCR) for typing strains belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii complex and application of the method to the investigation of a hospital outbreak.基于全细胞重复基因外回文序列聚合酶链反应（REP-PCR）对醋酸钙不动杆菌-鲍曼不动杆菌复合体菌株进行分型的验证及该方法在医院感染暴发调查中的应用

J Clin Microbiol. 1996 May;34(5):1193-202. doi: 10.1128/jcm.34.5.1193-1202.1996.

本文引用的文献

DNA sequence analysis of revertants of the hisD3052 allele of Salmonella typhimurium TA98 using the polymerase chain reaction and direct sequencing: application to 1-nitropyrene-induced revertants.

Mutat Res. 1991 Feb;252(1):35-44. doi: 10.1016/0165-1161(91)90249-8.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验