Center for NanoBio Integration, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Analyst. 2009 Mar;134(3):577-81. doi: 10.1039/b813936a. Epub 2008 Dec 8.
A new silica-organic, double-network hydrogel was prepared from a mixture of silica nanoparticles, sodium silicate, and acrylamide to enable biomolecule encapsulation in a one-pot reaction, overcoming the brittleness of the silica matrix. The optimal composition of the hydrogel was 1:4 (v/v) acrylamide:silicate. The mechanical strength of the double-network hydrogel was almost twice that of a hydrogel prepared from only silica hydrogel. As a result of the hydrogel's increased strength, biomolecules encapsulated within the hydrogel also exhibited improved activity and durability.Trypsin, pepsin, and urease were each encapsulated within double-network hydrogels, and these encapsulated biomolecules showed good enzymatic activities, which were retained for several months. Therefore, the hydrogel was suitable for encapsulation of biomolecules for preparation of biodevices, such as bioreactors and biosensors.
一种新型的硅基有机双网络水凝胶由硅纳米粒子、硅酸钠和丙烯酰胺混合制备，可在一锅反应中实现生物分子的封装，克服了硅基质的脆性。水凝胶的最佳组成比为 1:4（v/v）丙烯酰胺：硅酸钠。双网络水凝胶的机械强度几乎是仅由硅凝胶制备的水凝胶的两倍。由于水凝胶强度的增加，包封在水凝胶内的生物分子也表现出更好的活性和耐久性。胰蛋白酶、胃蛋白酶和脲酶都被包封在双网络水凝胶中，这些包封的生物分子显示出良好的酶活性，并且可以保持几个月。因此，该水凝胶适合用于封装生物分子以制备生物设备，如生物反应器和生物传感器。
