Yang Yunxia, Huang Guowei, Zhang Xumei, Liu Huan
School of Public Health, Tianjin Medical University, Tianjin 300070, China.
Wei Sheng Yan Jiu. 2008 Nov;37(6):671-4.
To investigate the effects of folic acid (FA) on neural cell apoptosis and Notch1 mRNA expression in the ischemic brain tissues of rats with brain infarction.
Forty-eight male SD(Sprague-Dawley)rats were randomly separated into 4 groups according to body mass: Sham operation (SO), middle cerebral artery occlusion (MCAO), MCAO + low dose folic acid (MCAO + LFA), and MCAO + high dose folic acid (MCAO + HFA). MCAO was made by operation with middle cerebral artery occlusion method except for SO, and the rats were executed at the 14th d after MCAO. Serum folic acid contents were measured with immune chemiluminescence before and 28d after folic acid supplementation and 14d after MCAO. Ischemic brain tissues neural cell apoptosis was measured with TUNEL method, Notch1 mRNA expression was detected by fluorescence in situ hybridization at the 14th d after the rats with MCAO.
In comparison with MCAO, the rates of neural cell apoptosis were more lower, the concentrations of serum folic acid and the fluorescence intensity of Notch1 mRNA were more higher than those in both MCAO + LFA and MCAO + HFA after FA supplementation (P < 0.01).
The rates of neural cell apoptosis induced by FA associated with the increase of Notch1 mRNA expression.
探讨叶酸（FA）对脑梗死大鼠缺血脑组织神经细胞凋亡及Notch1 mRNA表达的影响。
48只雄性SD（Sprague-Dawley）大鼠按体质量随机分为4组：假手术组（SO）、大脑中动脉闭塞组（MCAO）、MCAO+低剂量叶酸组（MCAO+LFA）和MCAO+高剂量叶酸组（MCAO+HFA）。除SO组外，其余组采用大脑中动脉闭塞法手术制备MCAO模型，于MCAO术后14 d处死大鼠。在补充叶酸前、补充叶酸后28 d及MCAO术后14 d，采用免疫化学发光法测定血清叶酸含量。采用TUNEL法检测缺血脑组织神经细胞凋亡，于MCAO术后14 d采用荧光原位杂交法检测Notch1 mRNA表达。
与MCAO组相比，补充叶酸后，MCAO+LFA组和MCAO+HFA组神经细胞凋亡率更低，血清叶酸浓度及Notch1 mRNA荧光强度更高（P<0.01）。
叶酸诱导的神经细胞凋亡率与Notch1 mRNA表达增加有关。
