Hu Mingqiu, Xin Dianqi, Chen Jun, Sun Guofeng, Wang Yiming, Na Yanqun
Department of Urology, Peking University First Hospital, Beijing, PR China.
BJU Int. 2009 Aug;104(3):406-11. doi: 10.1111/j.1464-410X.2009.08442.x. Epub 2009 Feb 23.
To characterize the changes in androgen levels in the prostate after castration, as androgens are critical in the progression of prostate cancer after castration, but the time at which the androgen remaining in the prostatic cancer tissue after castration exerts its effects is poorly understood.
The ventral prostate (VP) in adult male spontaneously hypertensive rats was excised at 2, 4 and 8 h, 1, 2, 4 and 7 days, and 2, 4 and 8 weeks after castration. The dihydrotestosterone (DHT), testosterone, dehydroepiandrosterone (DHEA) and androstenedione (4-dione) levels in the VP were measured simultaneously using gas chromatography/tandem mass spectrometry.
Within 2 days of castration, the DHT and testosterone levels in the VP decreased sharply, while there were no significant changes in the DHEA or 4-dione levels. From 2 days to 2 weeks after castration (2-7 days for 4-dione), there was a sharp peak in tissue androgen levels in the VP (P < 0.05 for all androgens); during the subsequent 6 weeks after castration, all of the tissue DHT, testosterone, DHEA and 4-dione levels gradually increased with time.
These data show the changes which occur in androgen levels in rat VP after castration and support the concept that the adrenal glands compensate for the loss of testicular androgen.
去势后前列腺中的雄激素水平会发生变化，雄激素在去势后前列腺癌进展中至关重要，但去势后前列腺癌组织中残留的雄激素发挥作用的时间却知之甚少，本研究旨在对此进行描述。
在成年雄性自发性高血压大鼠去势后的2、4和8小时、1、2、4和7天以及2、4和8周时，切除其腹侧前列腺（VP）。使用气相色谱/串联质谱法同时测量VP中的双氢睾酮（DHT）、睾酮、脱氢表雄酮（DHEA）和雄烯二酮（4-二酮）水平。
去势后2天内，VP中的DHT和睾酮水平急剧下降，而DHEA或4-二酮水平无显著变化。去势后2天至2周（4-二酮为2-7天），VP中的组织雄激素水平出现一个尖峰（所有雄激素P<0.05）；在去势后的后续6周内，所有组织中的DHT、睾酮、DHEA和4-二酮水平均随时间逐渐升高。
这些数据显示了大鼠VP去势后雄激素水平的变化，并支持肾上腺补偿睾丸雄激素损失这一概念。
