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Non-isotopic in situ hybridization with digoxigenin and alkaline phosphatase labelled oligodeoxynucleotide probes. Applications in pituitary gland.

作者信息

McNicol A M, Farquharson M A, Walker E

机构信息

University Department of Pathology, Royal Infirmary, Glasgow, U.K.

出版信息

Pathol Res Pract. 1991 Jun;187(5):556-8. doi: 10.1016/S0344-0338(11)80142-1.

DOI:10.1016/S0344-0338(11)80142-1
PMID:1923946
Abstract

We report the application of digoxigenin labelled oligonucleotide probes for the detection of hormonal messenger RNAs (mRNAs) in human pituitary adenomas. Positive signal for the appropriate mRNA was detected in tumours associated with Cushing's disease, acromegaly and hyperprolactinaemia, where immunoreactivity for adrenocorticotrophin (ACTH) growth hormone and prolactin had also been confirmed. In addition, we report the detection of proopiomelanocortin (POMC) mRNA in the rat pituitary gland using an oligodeoxynucleotide probe directly linked to alkaline phosphatase.

摘要

相似文献

1
Non-isotopic in situ hybridization with digoxigenin and alkaline phosphatase labelled oligodeoxynucleotide probes. Applications in pituitary gland.
Pathol Res Pract. 1991 Jun;187(5):556-8. doi: 10.1016/S0344-0338(11)80142-1.
2
Detection of messenger RNA using a digoxigenin end labelled oligodeoxynucleotide probe.使用地高辛配基末端标记的寡脱氧核苷酸探针检测信使核糖核酸
J Clin Pathol. 1990 May;43(5):424-8. doi: 10.1136/jcp.43.5.424.
3
Rapid in situ hybridization using digoxigenin probe and microwave oven.使用地高辛配体探针和微波炉进行快速原位杂交。
Cell Mol Biol (Noisy-le-grand). 1995 Nov;41(7):975-7.
4
Detection of mRNA by in situ hybridisation and in northern blot analysis using oligodeoxynucleotide probes labelled with alkaline phosphatase.使用碱性磷酸酶标记的寡脱氧核苷酸探针通过原位杂交和Northern印迹分析检测mRNA。
J Clin Pathol. 1992 Nov;45(11):999-1002. doi: 10.1136/jcp.45.11.999.
5
Detection of proopiomelanocortin mRNA by in situ hybridization, using a biotinylated oligodeoxynucleotide probe and avidin-alkaline phosphatase histochemistry.使用生物素化寡脱氧核苷酸探针和抗生物素蛋白-碱性磷酸酶组织化学，通过原位杂交检测阿黑皮素原mRNA。
Histochemistry. 1988;89(2):109-16. doi: 10.1007/BF00489913.
6
Changes in rat pituitary POMC mRNA after exposure to cold or a novel environment, detected by in situ hybridization.通过原位杂交检测大鼠暴露于寒冷或新环境后垂体前叶促肾上腺皮质激素原（POMC）mRNA的变化。
J Histochem Cytochem. 1991 Jun;39(6):843-52. doi: 10.1177/39.6.1851778.
7
Detection of growth hormone, prolactin and human beta-chorionic gonadotropin mRNA in growth hormone-secreting pituitary adenomas and in prolactin-secreting pituitary adenomas by in situ hybridization using a non-isotopic detection method.使用非同位素检测方法，通过原位杂交技术检测生长激素分泌型垂体腺瘤和催乳素分泌型垂体腺瘤中的生长激素、催乳素及人绒毛膜促性腺激素β亚基信使核糖核酸。
Acta Endocrinol (Copenh). 1993 May;128(5):411-7. doi: 10.1530/acta.0.1280411.
8
In situ hybridization histochemistry of mRNAs for hormones and chromogranins in normal pituitary tissue and pituitary adenoma.正常垂体组织和垂体腺瘤中激素及嗜铬粒蛋白mRNA的原位杂交组织化学
Acta Endocrinol (Copenh). 1991 Dec;125(6):628-36. doi: 10.1530/acta.0.1250628.
9
Analysis of endocrine active and clinically silent corticotropic adenomas by in situ hybridization.通过原位杂交分析内分泌活性及临床无症状的促肾上腺皮质激素腺瘤
Am J Pathol. 1990 Aug;137(2):479-88.
10
Effects of corticotropin-releasing hormone and dexamethasone on proopiomelanocortin messenger RNA level in human corticotroph adenoma cells in vitro.促肾上腺皮质激素释放激素和地塞米松对体外培养的人促肾上腺皮质激素腺瘤细胞中阿黑皮素原信使核糖核酸水平的影响。
J Clin Invest. 1988 Jul;82(1):110-4. doi: 10.1172/JCI113557.

引用本文的文献

1
Combined non-isotopic in situ hybridisation and indirect immunohistochemical analysis of hormone production in the rat pituitary gland.大鼠垂体中激素产生的联合非同位素原位杂交与间接免疫组织化学分析
Clin Mol Pathol. 1996 Feb;49(1):M57-60. doi: 10.1136/mp.49.1.m57.
2
Sensitive detection of human growth hormone mRNA in routinely formalin-fixed, paraffin-embedded transgenic mouse tissues by non-isotopic in situ hybridization.通过非同位素原位杂交技术灵敏检测常规福尔马林固定、石蜡包埋的转基因小鼠组织中的人生长激素mRNA
Histochemistry. 1994 Aug;102(2):145-52. doi: 10.1007/BF00269018.
3
The effects of varying key steps in the non-radioactive in situ hybridization protocol: a quantitative study.
非放射性原位杂交实验方案中不同关键步骤的效果：一项定量研究
Histochem J. 1995 Jan;27(1):60-8. doi: 10.1007/BF00164173.