Chen Jau-Shiuh, Chiu Hsien-Ching, Hsu Chih-Jung, Liu Ching-Yi, Hsieh Pei-Chen, Miaw Shi-Chuen, Yu Hsin-Su, Wang Li-Fang
Department of Dermatology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
J Invest Dermatol. 2009 Sep;129(9):2258-64. doi: 10.1038/jid.2009.24. Epub 2009 Feb 26.
Epicutaneous sensitization has been an important route for protein allergen sensitization in atopic disease. Although the skin is irradiated by sunlight daily, the influence of visible light on epicutaneous sensitization has not been explored. In this study, by using a well-established murine protein-patch model, we show that low-energy visible light (LEVL) irradiation could differentially modulate the predominant Th2 immune response induced by epicutaneous sensitization with protein antigen. When the induced Th2 response was strong, as usually observed in BALB/c mice, LEVL irradiation suppressed the response. In contrast, LEVL irradiation enhanced the weaker Th2 response in C57BL/6 mice. Increased IL-18 and decreased TGF-beta expression in draining lymph nodes after LEVL irradiation was observed in BALB/c mice, but not in C57BL/6 mice. LEVL irradiation also enhanced IL-18 expression in skin and reduced the downregulation of CD24 expression on epidermal Langerhans cells in draining lymph nodes of BALB/c mice. Collectively, these results provide evidence for immunomodulatory effects of LEVL irradiation and will help us develop a useful strategy for prevention of allergen sensitization.
表皮致敏一直是特应性疾病中蛋白质过敏原致敏的重要途径。尽管皮肤每天都受到阳光照射，但可见光对表皮致敏的影响尚未得到研究。在本研究中，通过使用成熟的小鼠蛋白质贴片模型，我们发现低能量可见光（LEVL）照射可差异性地调节由蛋白质抗原表皮致敏诱导的主要Th2免疫反应。当诱导的Th2反应较强时，如在BALB/c小鼠中通常观察到的那样，LEVL照射会抑制该反应。相反，LEVL照射增强了C57BL/6小鼠中较弱的Th2反应。在BALB/c小鼠中观察到LEVL照射后引流淋巴结中IL-18增加且TGF-β表达降低，但在C57BL/6小鼠中未观察到。LEVL照射还增强了皮肤中IL-18的表达，并减少了BALB/c小鼠引流淋巴结中表皮朗格汉斯细胞上CD24表达的下调。总体而言，这些结果为LEVL照射的免疫调节作用提供了证据，并将有助于我们制定预防过敏原致敏的有用策略。
