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通过酶联免疫吸附测定法(ELISA)测定新生犊牛出生后至第10天血清中IgG和IgM水平,并描述其与血浆总蛋白浓度和γ-谷氨酰转移酶(GGT)活性的相关性。

Determination of IgG and IgM levels in sera of newborn calves until the 10th day of life by ELISA and description of their correlation to total plasma protein concentration and GGT activity.

作者信息

Bender Petra, Bostedt Hartwig

机构信息

Clinic for Obstetrics, Gynaecology and Andrology of Large and Small Animals, Justus Liebig University, Giessen, Germany.

出版信息

Dtsch Tierarztl Wochenschr. 2009 Feb;116(2):44-52.

Abstract

The aim of this study was the determination of IgG and IgM concentrations in sera of 15 vital and healthy calves from the day of birth to the 10th day of life using two ELISAs exclusively developed for this purpose. We investigated if and to which extent the sera profiles were correlated with antibody levels in the colostral milk administered, with GGT activity and with total plasma protein content. Due to the assays' high sensitivity, traces of IgG and IgM in calf sera could be determined prior to the first uptake of the foremilk. Throughout the colostrum administration period until the 12th living hour, IgG and IgM levels remarkably increased (P < 0.0001).The correlation between IgG concentrations in sera determined 24 h post natum and the IgG content of the colostrum administered was highly significant (P < 0.001; r = 0.851), while the correlation of seral IgM levels 24 h post natum and the IgM content of the foremilk was significant (P = 0.009; r = 0.651). The sum of the IgG and IgM concentrations in calf serum 24 h post natum was significantly correlated with the neonatal plasma protein level (P = 0.01; r = 0.642). With P = 0.012; r = 0.629 and P = 0.029; r = 0.561 respectively, there was also a significant correlation between the subjects' IgG and IgM concentrations at 24 h post natum and the GGT activity in calf serum. By looking at individual cases, it became evident that the administration of colostrum containing maximum or minimum immunoglobulin concentrations does not necessarily result in the respective sera immunoglobulin concentrations. From these findings, as well as from the fact that numerous subjects displayed their highest IgG and IgM sera concentrations well after the gut closure, we conclude that individually diverse resorption patterns are in place which cannot be characterized by immunoglobulin measurements only. The determination of the total plasma protein content or GGT activity in calf serum at 24 h post natum only give a rough idea about the actual immunoglobulin supply of the calves, since for the individual subject no conclusion could be drawn to the extent of immunoglobulin concentrations.

摘要

本研究的目的是使用专门为此开发的两种酶联免疫吸附测定法(ELISA),测定15头健康新生犊牛从出生当天到出生后第10天血清中的IgG和IgM浓度。我们调查了血清谱是否以及在何种程度上与所喂初乳中的抗体水平、γ-谷氨酰转移酶(GGT)活性以及血浆总蛋白含量相关。由于检测方法的高灵敏度,在犊牛首次摄取初乳前就能检测到血清中痕量的IgG和IgM。在整个初乳投喂期直至出生后12小时,IgG和IgM水平显著升高(P<0.0001)。出生后24小时测定的血清IgG浓度与所喂初乳的IgG含量之间的相关性非常显著(P<0.001;r=0.851),而出生后24小时血清IgM水平与初乳IgM含量的相关性显著(P=0.009;r=0.651)。出生后24小时犊牛血清中IgG和IgM浓度之和与新生血浆蛋白水平显著相关(P=0.01;r=0.642)。出生后24小时犊牛血清中IgG和IgM浓度与犊牛血清GGT活性之间也分别存在显著相关性,P值分别为0.012;r=0.629和P=0.029;r=0.561。通过观察个体病例发现,投喂免疫球蛋白浓度最高或最低的初乳并不一定导致相应的血清免疫球蛋白浓度。从这些发现以及许多个体在肠道关闭后血清IgG和IgM浓度才达到最高这一事实,我们得出结论,存在个体差异的吸收模式,仅通过免疫球蛋白测量无法对其进行表征。出生后24小时测定犊牛血清中的血浆总蛋白含量或GGT活性,只能大致了解犊牛实际的免疫球蛋白供应情况,因为对于个体而言,无法根据这些指标推断免疫球蛋白浓度的具体情况。

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