Vann R C, Holloway J W, Carstens G E, Boyd M E, Randel R D
Texas Agricultural Experiment Station, Overton 75684, USA.
J Anim Sci. 1995 Oct;73(10):3044-50. doi: 10.2527/1995.73103044x.
Purebred Bos indicus calves are documented to have lower survival rates than Bos taurus calves. Thus, this study was designed to investigate the possibility that this decreased survival rate may be attributed to dam colostral immunoglobulin (Ig) concentrations and subsequent calf serum Ig concentrations. The specific objective was to determine the effect of breed type of calf on colostrum production, immunoglobulin concentrations in colostrum and calf serum, and availability and absorption efficiency of Ig. Brahman (B) and Angus (A) cattle were reciprocally mated to produce calves of the following types: A x A (n = 8), A x B (n = 9), B x B (n = 11), and B x A (n = 11). At birth, calves were separated from their dams and a blood sample was collected before feeding pooled colostrum (30 mL/kg birth weight) at 1 and 6 h of age. From 6 to 12 h of age, each calf was placed in a box that allowed interaction with the dam but prevented suckling. At 12 h of age, each calf was fed its dam's colostrum and placed with the dam. Additional blood samples were collected at 12, 24, and 48 h after birth. Serum and colostrum samples were analyzed for IgG, IgG1, IgG2, IgM, and IgA using single radial immunodiffusion (RID) assay techniques. The cows were hand-milked after induction of milk letdown with oxytocin at 1 and 12 h after calving. Colostrum volume was recorded, and samples were collected. Brahman cows produced more (P < .001) colostrum at 1 and 12 h than A cows. Total Ig concentrations were obtained by summing IgG, IgG1, IgG2, IgM, and IgA concentrations. Total Ig (P < .02), IgG (P < .005), and IgA (P < .01) concentrations in colostrum were greater in cows producing crossbred calves. Total Ig (P < .006), IgG (P < .02), IgG1 (P < .004), and IgG2 (P < .02) available in colostrum were affected by B x B and A x B breed types of calf. Brahman cows had more Ig available at 1 and 12 h than A cows due to increased production of colostrum. Breed type influenced colostral Ig in cattle. Serum concentrations of total Ig, IgG, IgG1, IgG2, IgM, and IgA in the calf and efficiency of absorption at 6 and 12 h were not affected by breed type, sex of calf, or any interaction.
有文献记载，纯种印度瘤牛犊牛的存活率低于普通牛犊牛。因此，本研究旨在探讨存活率降低是否可能归因于母牛初乳免疫球蛋白（Ig）浓度以及随后的犊牛血清Ig浓度。具体目标是确定犊牛品种类型对初乳产量、初乳和犊牛血清中的免疫球蛋白浓度以及Ig的可利用性和吸收效率的影响。婆罗门牛（B）和安格斯牛（A）进行正反交，以产生以下类型的犊牛：A×A（n = 8）、A×B（n = 9）、B×B（n = 11）和B×A（n = 11）。出生时，将犊牛与其母牛分开，并在1小时和6小时龄饲喂混合初乳（30 mL/千克出生体重）之前采集血样。在6至12小时龄时，将每头犊牛放入一个盒子中，使其能够与母牛互动但防止哺乳。在12小时龄时，每头犊牛饲喂其母牛的初乳并与母牛放在一起。出生后12、24和48小时采集额外的血样。使用单向辐射免疫扩散（RID）分析技术分析血清和初乳样品中的IgG、IgG1、IgG2、IgM和IgA。在产犊后1小时和12小时用催产素诱导排乳后，对母牛进行手工挤奶。记录初乳体积并采集样品。婆罗门母牛在1小时和12小时时分泌的初乳比安格斯母牛多（P < .001）。初乳中的总Ig浓度通过将IgG、IgG1、IgG2、IgM和IgA浓度相加获得。产杂交犊牛的母牛初乳中的总Ig（P < .02）﹑IgG（P < .005）和IgA（P < .01）浓度更高。初乳中可利用的总Ig（P < .006）、IgG（P < .02）、IgG1（P < .004）和IgG2（P < .02）受B×B和A×B犊牛品种类型的影响。由于初乳产量增加，婆罗门母牛在1小时和12小时时的可利用Ig比安格斯母牛多。品种类型影响牛的初乳Ig。犊牛血清中总Ig、IgG、IgG1、IgG2、IgM和IgA的浓度以及6小时和12小时时的吸收效率不受品种类型、犊牛性别或任何相互作用的影响。
