来自松鼠（Sciurus niger）（啮齿目：松鼠科）的艾美耳球虫（顶复门：艾美耳科）种类及内转录间隔区1（ITS1）、内转录间隔区2（ITS2）和5.8S核糖体DNA分析
Species of Eimeria (Apicomplexa: Eimeriidae) from tree squirrels (Sciurus niger) (Rodentia: Sciuridae) and analysis of the ITS1, ITS2, and 5.8S rDNA.
作者信息
Motriuk-Smith Dagmara, Seville R Scott, Oliver Clinton E, Hofmann Danielle L, Smith Arik W
机构信息
Department of Zoology and Physiology, University of Wyoming, Casper, Wyoming 82601, USA.
出版信息
J Parasitol. 2009 Feb;95(1):191-7. doi: 10.1645/GE-1653.1.
During the winter of 2004, 48 fecal samples were collected from live-trapped fox squirrels (Sciurus niger) from central Wyoming (Natrona County) and examined for species of Eimeria. Two species, Eimeria lancasterensis (prevalence, 65%) and Eimeria ontarioensis (prevalence, 27%), were identified. Genomic DNA sequences ITS1 and ITS2 were amplified, cloned, and sequenced. Additional sequences from E. lancasterensis isolated from a Delmarva fox squirrel (Sciurus niger cinereus) collected on Chincoteague Island, Virginia, were also identified. Comparison of pairwise distances suggests that E. lancasterensis from Wyoming and Virginia are conspecific. Maximum Parsimony tree construction identified 2 lineages, one E. ontarioensis and one E. lancasterensis; and both lineages had a strong bootstrap support (100%). The Maximum Parsimony analysis was unable to resolve the Wyoming and Virginia strains.
2004年冬季，从怀俄明州中部（纳特罗纳县）通过活捉诱捕的狐松鼠（Sciurus niger）身上采集了48份粪便样本，用于检查艾美耳球虫的种类。鉴定出了两种球虫，即兰卡斯特艾美耳球虫（感染率65%）和安大略艾美耳球虫（感染率27%）。对核糖体DNA内转录间隔区1（ITS1）和内转录间隔区2（ITS2）的基因组DNA序列进行了扩增、克隆和测序。还鉴定了从弗吉尼亚州钦科蒂格岛采集的一只戴尔马瓦狐松鼠（Sciurus niger cinereus）身上分离出的兰卡斯特艾美耳球虫的其他序列。成对距离比较表明，来自怀俄明州和弗吉尼亚州的兰卡斯特艾美耳球虫是同种的。最大简约树构建确定了两个谱系，一个是安大略艾美耳球虫谱系，另一个是兰卡斯特艾美耳球虫谱系；两个谱系都有很强的自展支持率（100%）。最大简约分析无法区分来自怀俄明州和弗吉尼亚州的菌株。
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