[双侧腹腔隐睾症中富集胎儿细胞培养物异种移植时睾丸组织的再生及大鼠生育能力的恢复]

[Regeneration of testicular tissue and restoration of rat fertility in xenotransplantation of enriched fetal cell cultures in bilateral abdominal cryptorchism].

作者信息

Kamalov A A, Gukhikh G T, Kirpatovskiĭ V I, Zaraĭskiĭ E I, Poltavtseva R A, Plotnikov E Iu, Kudriavtsev Iu V, Efremov E A, Obokhotov D A

出版信息

Urologiia. 2008 Nov-Dec(6):7-11.

PMID:19248591

Abstract

The study of cell cultures enriched with stem and progenitor cells in the treatment of experimental hypergonadotropic hypogonadism was made on 30 white non-inbred rats with experimental cryptorchism who have undergone xenotransplantation of human fetal enriched cell cultures. Spermatogenic epithelium on histological sections was studied on day 14 and 28 after xenotransplantation with calculation of the spermatogenesis index. The fertility index was estimated for each of the groups. Transplantation of enriched cell cultures enhances efficacy of restoration of adequate germinogenic and spermatogenic testicular function, accelerates recovery of spermatogenesis and fertility with high indices of spermatogenesis and fertility but effect of the above recovery after treatment with enriched cell cultures can be seen not earlier than 72 days after transplantation.

摘要

对30只患有实验性隐睾症的白色非近交系大鼠进行了富含干细胞和祖细胞的细胞培养物治疗实验性高促性腺激素性性腺功能减退的研究，这些大鼠接受了人胎儿富集细胞培养物的异种移植。在异种移植后第14天和第28天对组织学切片上的生精上皮进行研究，并计算精子发生指数。对每组大鼠的生育指数进行评估。富集细胞培养物的移植提高了恢复适当的生精和睾丸生精功能的疗效，加速了精子发生和生育能力的恢复，精子发生和生育指数较高，但富集细胞培养物治疗后的上述恢复效果在移植后不早于72天才能显现。