Price P, Bush C, Parkins C S, McMillan T J, Robinson M, Steel G G
Radiotherapy Research Unit, Institute of Cancer Research, Sutton, Surrey, U.K.
Radiother Oncol. 1991 Aug;21(4):282-5. doi: 10.1016/0167-8140(91)90055-l.
Baker et al. [Cancer Res. 46: 1263-1274, 1986] developed an adhesive tumour cell culture system (ATCCS) using a culture surface coated with a cell attachment matrix (CAM). The ability of CAM-coated plates to support the growth of cells from human tumour biopsies has been evaluated. Successful growth was obtained in 9/22 samples (41%), but fibroblasts, rather than tumour cells, grew in the majority. Comparison of CAM with other surfaces showed that CAM was no better for establishing tumour cell growth than the presence of feeder cells or an alternative attachment factor vitronectin.
贝克等人[《癌症研究》46: 1263 - 1274, 1986]开发了一种黏附性肿瘤细胞培养系统（ATCCS），该系统使用涂有细胞附着基质（CAM）的培养表面。已对涂有CAM的培养板支持人肿瘤活检细胞生长的能力进行了评估。在22个样本中有9个（41%）成功实现了生长，但大多数情况下生长的是成纤维细胞而非肿瘤细胞。将CAM与其他表面进行比较表明，在促进肿瘤细胞生长方面，CAM并不比饲养细胞或替代附着因子玻连蛋白更好。
