Wang Steven S-S, Liu Kuan-Nan, Lu Yen-Chun
Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
Biochem Biophys Res Commun. 2009 Apr 17;381(4):639-42. doi: 10.1016/j.bbrc.2009.02.103. Epub 2009 Feb 25.
This work examines the inhibitory effect of TCEP on the in vitro fibrillation of hen lysozyme at pH 2. We demonstrate that the inhibition of hen lysozyme fibrillation by TCEP follows a dose-dependent manner. Our data show that the addition of TCEP prevents alpha-to-beta transition and promoted unfolding of lysozyme. Moreover, our findings suggested that the TCEP-induced attenuated fibrillation is associated with disulfide disruption and structural unfolding of HEWL.
这项工作研究了三（2-羧乙基）膦（TCEP）在pH 2条件下对鸡卵清溶菌酶体外纤维化的抑制作用。我们证明，TCEP对鸡卵清溶菌酶纤维化的抑制呈剂量依赖性。我们的数据表明，添加TCEP可防止α-到β-的转变并促进溶菌酶的去折叠。此外，我们的研究结果表明，TCEP诱导的纤维化减弱与溶菌酶的二硫键破坏和结构去折叠有关。
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