谷胱甘肽对鸡卵清白蛋白溶菌酶淀粉样纤维形成的影响。

Effects of glutathione on amyloid fibrillation of hen egg-white lysozyme.

机构信息

Department of Chemical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan.

出版信息

Int J Biol Macromol. 2009 Nov 1;45(4):321-9. doi: 10.1016/j.ijbiomac.2009.08.003. Epub 2009 Aug 21.

DOI:10.1016/j.ijbiomac.2009.08.003

PMID:19699759

Abstract

This study examined the effects of glutathione on the fibrillation of hen egg-white lysozyme. We found that the fibrillation of lysozyme was considerably reduced by GSH while no anti-aggregating activity was detected with only GSSG. SDS-PAGE results also revealed that the addition of GSH led to an early occurrence of prominent lysozyme hydrolysis. Moreover, GSH was effective in inhibiting lysozyme fibrillation when GSH was added within 6 days of incubation. We conclude that the attenuation of lysozyme fibrillation is strongly dependent upon the redox environment. Our data may contribute to decipher the molecular mechanism of amyloid fibrillation.

摘要

本研究考察了谷胱甘肽对鸡卵清溶菌酶纤维化的影响。我们发现，GSH 可显著减少溶菌酶的纤维化，而仅 GSSG 则没有抗聚集活性。SDS-PAGE 结果还表明，添加 GSH 会导致溶菌酶明显水解的早期发生。此外，当孵育 6 天内加入 GSH 时，GSH 可有效抑制溶菌酶纤维化。我们得出结论，溶菌酶纤维化的减弱强烈依赖于氧化还原环境。我们的数据可能有助于解析淀粉样纤维形成的分子机制。