Schreinemachers M C J M, Doorschodt B M, Florquin S, Idu M M, Tolba R H, van Gulik T M
Surgical Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
Transplant Proc. 2009 Jan-Feb;41(1):32-5. doi: 10.1016/j.transproceed.2008.08.146.
We sought to assess the efficacy of POLYSOL, a low-viscosity, colloid-based organ preservation solution, for the preservation of warm ischemically damaged kidney grafts compared with histidine-tryptophane-ketoglutarate (HTK) solution.
Pigs (25-30 kg) underwent a left nephrectomy after clamping the renal vessels for 30 minutes. Kidney grafts washed out with Polysol (n = 6) or HTK (n = 6) were cold stored (CS) for 20 hours at 4 degrees C. After the preservation period, the contralateral kidney was removed and the preserved kidney implanted heterotopically. Renal function was assessed daily for 7 days. Thereafter, animals were killed and the kidney grafts removed for histologic analysis.
All animals survived for 7 days. All Polysol CS-preserved grafts showed immediate function, as demonstrated by urine production within 24 hours after reperfusion as compared with 3/6 grafts in the HTK CS group. Overall, the Polysol CS group showed improved renal function compared with HTK CS. Also, peak serum creatinine and blood urea values were lower in the Polysol CS group compared with HTK-preserved grafts. Histologic evaluation of warm ischemically damaged grafts showed less glomerular shrinking, less tubular damage, less edema, less inflammatory infiltration, and less necrosis in Polysol compared with HTK-preserved grafts.
Application of Polysol solution for washout and CS preservation of warm ischemically damaged kidney grafts resulted in improved renal function and structural integrity when compared with HTK.
我们试图评估一种低粘度、基于胶体的器官保存溶液POLYSOL与组氨酸-色氨酸-酮戊二酸（HTK）溶液相比，对热缺血损伤的肾移植器官的保存效果。
对体重25-30千克的猪，在夹闭肾血管30分钟后进行左肾切除术。用POLYSOL（n = 6）或HTK（n = 6）冲洗后的肾移植器官在4℃下冷藏（CS）20小时。保存期过后，切除对侧肾脏，并将保存的肾脏异位植入。连续7天每天评估肾功能。此后，处死动物并取出肾移植器官进行组织学分析。
所有动物均存活7天。所有经POLYSOL冷藏保存的移植器官均显示立即发挥功能，再灌注后24小时内即有尿液生成，而HTK冷藏组6个移植器官中只有3个如此。总体而言，与HTK冷藏组相比，POLYSOL冷藏组的肾功能有所改善。此外，与HTK保存的移植器官相比，POLYSOL冷藏组的血清肌酐峰值和血尿素值更低。对热缺血损伤的移植器官进行组织学评估发现，与HTK保存的移植器官相比，POLYSOL保存的移植器官肾小球萎缩、肾小管损伤、水肿、炎症浸润和坏死均较少。
与HTK相比，应用POLYSOL溶液冲洗并冷藏保存热缺血损伤的肾移植器官可改善肾功能和结构完整性。
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