用于肾移植冲洗的保存液比较：一项实验研究。

Comparison of preservation solutions for washout of kidney grafts: an experimental study.

作者信息

Schreinemachers M C J M, Doorschodt B M, Florquin S, Tolba R H

机构信息

Surgical Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.

出版信息

Transplant Proc. 2009 Dec;41(10):4072-9. doi: 10.1016/j.transproceed.2009.09.089.

DOI:10.1016/j.transproceed.2009.09.089

PMID:20005343

Abstract

OBJECTIVE

The impact of different preservation solutions for washout of kidney grafts was evaluated regarding temperature, kidney weight, remaining red blood cells (RBCs) and histological evaluation after ex vivo washout using 500 mL cold preservation solution at 4 degrees C followed by 24 hours cold storage (CS).

METHODS

Kidneys retrieved from Landrace pigs (20-30 kg) were immediately washed (warm ischemic time 0 min [WIT 0]), using 500 mL cold University of Wisconsin solution (UW), histidine-tryptophan-ketoglutarate (HTK), or Polysol (PS) followed by 24 hours, CS. Also, kidneys were retrieved after a WIT of 30 minutes followed by washout using HTK or PS.

RESULTS

After washout, the weight of kidneys washed out with HTK had increased, whereas that of organs in the UW or PS group had decreased. After washout with UW, the core temperature of WIT 0 kidneys was lower than that with HTK. The time needed for washout using 500 mL solution was shorter using PS compared with HTK for both WIT 0 and WIT 30 groups. The amount of remaining RBCs was similar between all WIT 0 groups; whereas in the WIT 30 groups the amount was higher in kidneys washed out using HTK compared with PS. Histological evaluation showed less tissue injury among PS-washed kidneys compared with UW or HTK.

CONCLUSION

Overall, kidneys washed-out with PS showed better preservation of structural integrity after 24 hours, CS compared with either UW or HTK. Washout of warm ischemically damaged kidneys was more effective using PS compared with HTK.

摘要

目的

在4℃下使用500 mL冷保存液进行离体冲洗后，接着进行24小时冷藏（CS），评估不同保存液对肾移植冲洗的影响，包括温度、肾重量、剩余红细胞（RBC）以及组织学评估。

方法

从长白猪（20 - 30 kg）获取的肾脏立即进行冲洗（热缺血时间0分钟[WIT 0]），使用500 mL冷的威斯康星大学溶液（UW）、组氨酸 - 色氨酸 - 酮戊二酸（HTK）或聚醇（PS），随后进行24小时CS。此外，在热缺血30分钟后获取肾脏，接着用HTK或PS进行冲洗。

结果

冲洗后，用HTK冲洗的肾脏重量增加，而UW或PS组器官的重量减少。用UW冲洗后，WIT 0肾脏的核心温度低于用HTK冲洗的。对于WIT 0和WIT 30组，使用500 mL溶液冲洗时，PS所需时间比HTK短。所有WIT 0组之间剩余RBC的量相似；而在WIT 30组中，与PS相比，用HTK冲洗的肾脏中RBC量更高。组织学评估显示，与UW或HTK相比，PS冲洗的肾脏组织损伤更少。