Garrastazu C, Giménez M J, Iniesta M P, Aránguez M I, Ruiz-Amil M
Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Rev Esp Fisiol. 1991 Jun;47(2):63-8.
Propionyl-CoA synthetase of liver and mammary gland from calf and midlactation cow was investigated. No activity of this enzyme was detected in calf mammary gland, but it was detected in calf liver. Propionyl-CoA synthetase was found in both, liver and mammary gland of the cow, although mammary gland activity was about 25% of that found in liver. The effects of pH and temperature on enzyme activity and stability were also investigated in crude extracts of liver and mammary gland tissues. The results suggest a different behaviour of the enzyme from both origins. Kinetic studies of the enzyme were also carried out, showing differences, depending on the organ, in the apparent substrate KM values.
对小牛和泌乳中期奶牛肝脏及乳腺中的丙酰辅酶A合成酶进行了研究。在小牛乳腺中未检测到该酶的活性，但在小牛肝脏中检测到了。在奶牛的肝脏和乳腺中均发现了丙酰辅酶A合成酶，尽管乳腺中的活性约为肝脏中的25%。还研究了pH值和温度对肝脏和乳腺组织粗提物中酶活性及稳定性的影响。结果表明，来自这两种来源的酶表现出不同的特性。还对该酶进行了动力学研究，结果表明，根据器官不同，其表观底物KM值存在差异。
