Rocha Lílian Alves, Ferreira de Almeida E Borges Lizandra, Gontijo Filho Paulo Pinto
Imunologia e Parasitologia Aplicadas, Programa de Pós Graduação em Imunologia e Parasitologia Aplicadas, Universidade Federal de Uberlândia, Minas Gerais, Brazil.
Am J Infect Control. 2009 Mar;37(2):155-9. doi: 10.1016/j.ajic.2008.04.251.
The purpose of this study was evaluating the microbial flora of nurses' healthy (n = 30) and damaged hand (n = 30) by frequent handwashing and/or wearing of gloves.
Hand cultures were obtained both before and after washing hands with nonantimicrobial soap, through the sterile polyethylene bag method.
The bacteria counts of the hands of professionals with damaged hands were higher than those with healthy hands, and those with damaged hands presented higher frequency of Staphylococcus aureus, 16.7% versus 10%; gram-negative bacteria, 20% versus 6.7%; and yeast, 26.7% versus 20%, respectively, as well as the sum of these microorganisms. The presence of Staphylococcus haemolyticus was only seen in nurses with damaged hands (P = .02), and enterococci were not recovered from the hands of any volunteer. The presence of antimicrobial-resistant S aureus and gram-negative bacteria was also greater among damaged hands.
The irritation caused on the skin by frequent washing and/or wearing of gloves is associated with changes in hands microbial flora, and their potential risks should be considered when institutions/users are selecting products/formulations to assure hands skin health and consequent compliance with their own hygiene procedures.
本研究旨在评估因频繁洗手和/或戴手套导致手部健康的护士（n = 30）和手部受损的护士（n = 30）的微生物菌群。
通过无菌聚乙烯袋法，在使用非抗菌肥皂洗手前后获取手部培养物。
手部受损的专业人员手部细菌计数高于手部健康者，手部受损者金黄色葡萄球菌、革兰氏阴性菌和酵母菌的出现频率分别更高，即金黄色葡萄球菌为16.7%对10%；革兰氏阴性菌为20%对6.7%；酵母菌为26.7%对20%，以及这些微生物的总和。溶血葡萄球菌仅在手部受损的护士中出现（P = 0.02），且未从任何志愿者的手部分离出肠球菌。手部受损者中耐抗菌药物的金黄色葡萄球菌和革兰氏阴性菌的出现情况也更多。
频繁洗手和/或戴手套对皮肤造成的刺激与手部微生物菌群的变化有关，在机构/使用者选择产品/配方以确保手部皮肤健康并进而遵守自身卫生程序时，应考虑其潜在风险。
