在大肠杆菌染色体复制起始时形成的复合物中，仅回收得到oriC及其侧翼区域。

Only oriC and its flanking region are recovered from the complex formed at the time of initiation of chromosome replication in Escherichia coli.

作者信息

Kataoka T, Gayama S, Takahashi K, Wachi M, Yamasaki M, Nagai K

机构信息

Department of Agricultural Chemistry, University of Tokyo.

出版信息

Res Microbiol. 1991 Feb-Apr;142(2-3):155-9. doi: 10.1016/0923-2508(91)90024-5.

DOI:10.1016/0923-2508(91)90024-5

PMID:1925014

Abstract

The oriC region of Escherichia coli constructs a specific complex to associate with the outer membrane fraction (oriC complex). The oriC complex was periodically formed before as well as in the short period after initiation of DNA replication. Using the DNA extracted from outer membrane fractions of the cells just after initiation as a probe, the whole E. coli genomic library was assayed by plaque hybridization. DNA regions that hybridized with the probe corresponded to about a 100-kb length of chromosome DNA which included oriC. In addition, clones located in a counterclockwise direction from oriC were more preferentially hybridized among these positive clones. Thus, we conclude that the oriC region is a unique locus of the chromosome that binds to the outer membrane at the time of initiation of chromosome replication.

摘要

大肠杆菌的oriC区域构建了一种与外膜组分相关的特异性复合物（oriC复合物）。oriC复合物在DNA复制起始之前以及起始后的短时间内周期性形成。以刚起始后细胞外膜组分中提取的DNA作为探针，通过噬菌斑杂交对整个大肠杆菌基因组文库进行检测。与探针杂交的DNA区域对应于约100kb长度的包含oriC的染色体DNA。此外，在这些阳性克隆中，位于从oriC逆时针方向的克隆更优先杂交。因此，我们得出结论，oriC区域是染色体的一个独特位点，在染色体复制起始时与外膜结合。