中枢神经系统中线细胞和表皮生长因子受体（Egfr）信号基因是果蝇中枢神经系统中RP2运动神经元谱系建立所必需的。

The CNS midline cells and Egfr signaling genes are required for establishment of the RP2 motoneuron lineage in the Drosophila central nervous system.

作者信息

Huh Jung Yun, Jeon Sang-Hak, Kim Sang Hee

机构信息

Department of Chemistry, Konkuk University, 1 Hwayang-dong Gwangjin-gu, Seoul 143-701, Republic of Korea.

出版信息

Biochem Biophys Res Commun. 2009 Mar 20;380(4):729-35. doi: 10.1016/j.bbrc.2009.01.104. Epub 2009 Jan 23.

DOI:10.1016/j.bbrc.2009.01.104

PMID:19250628

Abstract

It is well established that CNS midline cells are essential for the identity determination, division, and differentiation of neurons and glia in the Drosophila CNS. However, it is not clear whether CNS midline cells control the establishment and differentiation of the well-known RP2 motoneuron lineage. The present study showed by using several RP2 lineage markers that CNS midline cells and Egfr signaling genes are required for identity determination and formation of precursors of the RP2 motoneurons. Overexpression and ectopic expression of sim and components of the EGFR signaling pathway in the ventral neuroectoderm induced the formation of extra RP2s and their sibling cells by activating EGFR signaling. We demonstrated that CNS midline cells and Egfr signaling genes play essential roles in the establishment of the RP2 motoneuron lineage.

摘要

中枢神经系统中线细胞对于果蝇中枢神经系统中神经元和神经胶质细胞的身份确定、分裂和分化至关重要，这一点已得到充分证实。然而，尚不清楚中枢神经系统中线细胞是否控制着著名的RP2运动神经元谱系的建立和分化。本研究通过使用几种RP2谱系标记物表明，中枢神经系统中线细胞和表皮生长因子受体（Egfr）信号基因对于RP2运动神经元前体的身份确定和形成是必需的。在腹侧神经外胚层中过表达和异位表达sim和EGFR信号通路的成分，通过激活EGFR信号诱导了额外的RP2及其姐妹细胞的形成。我们证明了中枢神经系统中线细胞和Egfr信号基因在RP2运动神经元谱系的建立中起着至关重要的作用。