Vakili Abedin, Hosseinzadeh Seyed Ahmad, Khorasani Mahdi Zahedi
Laboratory of Cerebrovascular Research, Department and Research Center of Physiology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
J Stroke Cerebrovasc Dis. 2009 Mar-Apr;18(2):81-5. doi: 10.1016/j.jstrokecerebrovasdis.2008.09.018.
Carbenoxolone (CBX) has a neuroprotective effect in experimental models of brain ischemia and trauma. However, systemic effect of CBX on ischemic reperfusion injuries has not been investigated in a temporary model of focal cerebral ischemia. Male Wistar rats (n = 32) were divided into control and CBX-treated (100, 200, or 400 mg/kg, intraperitoneally) groups. Transient focal cerebral ischemia was induced by 60-minute middle cerebral artery occlusion by filament method, followed by 23-hour reperfusion. At the end of 24-hour ischemia, neurologic deficit score was tested and infarct volumes were determined using triphenyltetrazolium chloride staining. Administration of CBX (100, 200, or 400 mg/kg) at the beginning of ischemia significantly reduced cortical infarct volumes by 48%, 58%, and 63%, and striatal infarct volumes by 34%, 63%, and 63%, respectively. Nevertheless, CBX has no effect on neurologic dysfunction. Our findings indicated that peripheral administration of CBX has a neuroprotective effect on postischemic damage in a temporary model of focal cerebral ischemia in rat.
甘草次酸（CBX）在脑缺血和创伤的实验模型中具有神经保护作用。然而，在局灶性脑缺血的临时模型中，尚未研究CBX对缺血再灌注损伤的全身作用。将雄性Wistar大鼠（n = 32）分为对照组和CBX治疗组（腹腔注射100、200或400 mg/kg）。采用线栓法阻断大脑中动脉60分钟诱导短暂性局灶性脑缺血，随后进行23小时再灌注。在缺血24小时结束时，测试神经功能缺损评分，并使用氯化三苯基四氮唑染色法测定梗死体积。在缺血开始时给予CBX（100、200或400 mg/kg）可使皮质梗死体积分别显著减少48%、58%和63%，纹状体梗死体积分别显著减少34%、63%和63%。然而，CBX对神经功能障碍没有影响。我们的研究结果表明，在大鼠局灶性脑缺血的临时模型中，外周给予CBX对缺血后损伤具有神经保护作用。
