Castillo-Michel Hiram A, Zuverza-Mena Nubia, Parsons Jason G, Dokken Kenneth M, Duarte-Gardea Maria, Peralta-Videa Jose R, Gardea-Torresdey Jorge L
The University of Texas, El Paso, TX 79968-0513, USA.
Phytochemistry. 2009 Mar;70(4):540-5. doi: 10.1016/j.phytochem.2009.01.010. Epub 2009 Feb 27.
This study investigated the absorption of arsenic (As), sulfur (S), and phosphorus (P) in the desert plant Chilopsis linearis (Desert willow). A comparison between an inbred line (red flowered) and wild type (white flowered) plants was performed to look for differential responses to As treatment. One month old seedlings were treated for 7 days with arsenate (As(2)O(5), As(V)) at 0, 20, and 40 mg As(V)L(-1). Results from the ICP-OES analysis showed that at 20mg As(V)L(-1), red flowered plants had 280+/-11 and 98+/-7 mg As kg(-1) dry wt in roots and stems, respectively, while white flowered plants had 196+/-30 and 103+/-13 mg As kg(-1) dry wt for roots and stems. At this treatment level, the concentration of As in leaves was below detection limits for both plants. In red flowered plants treated with 40 mg As(V)L(-1), As was at 290+/-77 and 151+/-60 mg As kg(-1) in roots and stems, respectively, and not detected in leaves, whereas white flowered plants had 406+/-36, 213+/-12, and 177+/-40 mg As kg(-1) in roots, stems, and leaves. The concentration of S increased in all As treated plants, while the concentration of P decreased in roots and stems of both types of plants and in leaves of red flowered plants. X-ray absorption spectroscopy analyses demonstrated partial reduction of arsenate to arsenite in the form of As-(SX)(3) species in both types of plants.
本研究调查了沙漠植物线叶 Chilopsis（沙漠柳）对砷（As）、硫（S）和磷（P）的吸收情况。对一个自交系（红花）植株和野生型（白花）植株进行了比较，以寻找对砷处理的不同反应。用 0、20 和 40 mg As(V)L⁻¹ 的砷酸盐（As₂O₅，As(V)）处理 1 个月大的幼苗 7 天。电感耦合等离子体发射光谱（ICP - OES）分析结果表明，在 20mg As(V)L⁻¹ 时，红花植株根部和茎部的砷含量分别为 280±11 和 98±7 mg As kg⁻¹ 干重，而白花植株根部和茎部的砷含量分别为 196±30 和 103±13 mg As kg⁻¹ 干重。在此处理水平下，两种植株叶片中的砷浓度均低于检测限。在 40 mg As(V)L⁻¹ 处理的红花植株中，根部和茎部的砷含量分别为 290±77 和 151±60 mg As kg⁻¹，叶片中未检测到砷，而白花植株根部、茎部和叶片中的砷含量分别为 406±36、213±12 和 177±40 mg As kg⁻¹。所有经砷处理的植株中硫的浓度均增加，而两种类型植株的根部和茎部以及红花植株叶片中的磷浓度均降低。X 射线吸收光谱分析表明，两种类型的植株中砷酸盐均部分还原为亚砷酸盐，以 As-(SX)₃ 形式存在。
