Angulo-Valadez C E, Cepeda-Palacios R, Ascencio F, Jacquiet Ph, Dorchies Ph, Ramírez-Orduña J M, López M A
Centro de Investigaciones Biológicas del Noroeste, Mar Bermejo 195, Col. Playa de Santa Rita, La Paz, B.C.S. 23090, Mexico.
Vet Parasitol. 2009 May 12;161(3-4):356-9. doi: 10.1016/j.vetpar.2009.01.027. Epub 2009 Feb 6.
The aims of this study were to analyze the systemic IgG responses against third-instar salivary gland (L3SG) antigens by ELISA in Oestrus ovis experimentally infected kids (EIK) and in naturally exposed adult goats (NEG). Firstly, kids (n=4 per group) were assigned to receive intranasally 0, 12, 24, 36, and 48 first-instars in experimental infections. Blood samples were taken from EIK at Days 0, 14, 42 and 67 post-infection. At necropsy (Day 67), larval number and developmental instars were recorded. In an epidemiological study, blood serum samples were collected from 448 grazing NEG (n=20 flocks) in Baja California Sur, Mexico. Results showed that larval establishment rate was similar in EIK groups. Systemic IgG response reached the threshold after Day 42, but humoral response was not statistically different among EIK groups receiving experimental infections. In NEG, all surveyed flocks (100%) showed specific systemic IgG antibodies to L3SG antigens and the overall goat oestrosis prevalence was 59.2%. In conclusion, larval L3SG antigens were effective in detection of specific systemic IgG antibodies against O. ovis infected kids and goats by ELISA.
本研究旨在通过酶联免疫吸附测定（ELISA）分析实验性感染羊狂蝇的羔羊（EIK）和自然暴露的成年山羊（NEG）针对三期唾液腺（L3SG）抗原的全身性IgG反应。首先，将羔羊（每组n = 4）分配为在实验性感染中经鼻接受0、12、24、36和48只一期幼虫。在感染后第0、14、42和67天从EIK采集血样。在尸检时（第67天），记录幼虫数量和发育龄期。在一项流行病学研究中，从墨西哥南下加利福尼亚州的448只放牧NEG（n = 20群）采集血清样本。结果显示，EIK各实验组的幼虫定植率相似。全身性IgG反应在第42天后达到阈值，但接受实验性感染的EIK各实验组之间的体液反应在统计学上无差异。在NEG中，所有受调查的羊群（100%）均显示出针对L3SG抗原的特异性全身性IgG抗体，山羊狂蝇病的总体患病率为59.2%。总之，幼虫L3SG抗原通过ELISA能够有效检测感染羊狂蝇的羔羊和山羊体内的特异性全身性IgG抗体。
