体外旋转流条件下聚集介质中大鼠血液的超声背向散射

Ultrasonic backscatter from rat blood in aggregating media under in vitro rotational flow.

作者信息

Nam Kweon-Ho, Paeng Dong-Guk, Choi Min Joo

机构信息

Department of Bioengineering, University of Utah, Salt Lake City, UT, USA.

出版信息

IEEE Trans Ultrason Ferroelectr Freq Control. 2009 Feb;56(2):270-9. doi: 10.1109/TUFFC.2009.1036.

DOI:10.1109/TUFFC.2009.1036

PMID:19251514

Abstract

Ultrasonic backscatter from flowing and static rat red blood cells (RBCs) in autologous plasma and in 360 kDa polyvinylpyrrolidone (PVP 360) solution was measured as a function of hematocrit. The flow speed was varied by a stirring magnet in a cylindrical chamber. The radio-frequency (RF) signals backscattered by RBC samples were measured over 5 min in a pulse-echo setup with a 5 MHz focused transducer. Although the intact rat blood has poor RBC aggregability, RBC aggregation of rat blood was enhanced by replacing its plasma with a higher molecular weight polymer solution. The experimental results showed that the nonlinear relationship between hematocrit and ultrasonic backscatter from rat RBCs in plasma and aggregating media is affected by flow speed, which may provide a unified insight into hematocrit dependence of RBC aggregation under flowing and static conditions.

摘要

测量了自体血浆和360 kDa聚乙烯吡咯烷酮（PVP 360）溶液中流动和静态大鼠红细胞（RBC）的超声背向散射随血细胞比容的变化。通过圆柱形腔室中的搅拌磁体改变流速。在具有5 MHz聚焦换能器的脉冲回波设置中，在5分钟内测量RBC样品反向散射的射频（RF）信号。尽管完整的大鼠血液红细胞聚集性较差，但用高分子量聚合物溶液替代其血浆可增强大鼠血液的红细胞聚集。实验结果表明，血浆和聚集介质中大鼠红细胞的血细胞比容与超声背向散射之间的非线性关系受流速影响，这可能为流动和静态条件下红细胞聚集对血细胞比容的依赖性提供统一的见解。