Wang Yonggang, Zhu Fengrong, Chen Jie, Han Fusen, Wang Heyao
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
Biol Pharm Bull. 2009 Mar;32(3):389-93. doi: 10.1248/bpb.32.389.
Oxidative stress plays an important role in tissue damage associated with many different chronic degenerative diseases, such as cancer and cardiovascular disorders. In our previous study, a novel dipeptide Pro-Arg derived from protamine hydrolysates was found to possess significant antioxidative activity in vitro. In this study, we compared the antioxidant capabilities of purified and synthesized dipeptide (Pro-Arg) and found both of them had very strong hydroxyl-radical scavenging activity in vitro, even at a very low concentration. The protective effect of the dipeptide against oxidative stress was evaluated using H2O2-induced oxidative stress of human diploid fibroblasts MRC-5 cell model. Our results showed the dipeptide attenuated H2O2-induced cellular oxidative damage, and normalized S phase arrest of MRC-5 cells exposed to H2O2. Our findings demonstrate that Pro-Arg can protect against oxidative stress/damage and H2O2-induced human diploid fibroblasts cell death.
氧化应激在与许多不同慢性退行性疾病（如癌症和心血管疾病）相关的组织损伤中起重要作用。在我们之前的研究中，发现一种源自鱼精蛋白水解产物的新型二肽Pro-Arg在体外具有显著的抗氧化活性。在本研究中，我们比较了纯化和合成的二肽（Pro-Arg）的抗氧化能力，发现它们在体外即使在非常低的浓度下也具有很强的羟自由基清除活性。使用H2O2诱导的人二倍体成纤维细胞MRC-5细胞模型评估了该二肽对氧化应激的保护作用。我们的结果表明，该二肽减轻了H2O2诱导的细胞氧化损伤，并使暴露于H2O2的MRC-5细胞的S期阻滞恢复正常。我们的研究结果表明，Pro-Arg可以抵御氧化应激/损伤以及H2O2诱导的人二倍体成纤维细胞死亡。
